Antioxidant properties of the proteins caeruloplasmin, albumin and transferrin. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis

https://doi.org/10.1016/0167-4838(86)90286-4Get rights and content

Abstract

Patients with rheumatoid arthritis have altered protein patterns in their serum and synovial fluid which influences the antioxidant activity of these fluids. Rheumatoid serum has a higher antioxidant activity than control serum when ferrous and ferric ions stimulate membrane damage. The raised levels of caeruloplasmin and the lower iron saturation of transferrin contribute to these differences. When membrane damage is stimulated by a copper salt, rheumatoid serum does not show an increased antioxidant protection and has probably a lower protective activity than control serum. Attempts to damage caeruloplasmin and transferrin with oxygen radicals were unsuccessful. However, prolonged incubations with trypsin reduced the iron-binding capacity of transferrin and decreased the ferroxidase and antioxidant properties of caeruloplasmin. Copper was released from caeruloplasmin under these conditions.

References (40)

  • S. Marklund et al.

    Clin. Chim. Acta

    (1982)
  • D.A. Baldwin et al.

    J. Biol. Chem.

    (1984)
  • J.M.C. Gutteridge

    Biochem. Biophys. Res. Commun.

    (1977)
  • J.M.C. Gutteridge et al.

    FEBS Lett.

    (1980)
  • J.M.C. Gutteridge

    FEBS Lett.

    (1983)
  • J.M.C. Gutteridge et al.

    Clin. Chim. Acta

    (1984)
  • P. Carter

    Anal. Biochem.

    (1971)
  • G.W. Bates et al.

    J. Biol. Chem.

    (1967)
  • R.A. Lovstad

    Int. J. Biochem.

    (1982)
  • J.M.C. Gutteridge

    FEBS Lett.

    (1982)
  • P. Winyard et al.

    Int. J. Biochem.

    (1984)
  • L. Ryden

    FEBS Lett.

    (1971)
  • B.M. Babior

    N. Engl. J. Med.

    (1978)
  • B.M. Babior

    N. Engl. J. Med.

    (1978)
  • B. Halliwell et al.
  • I. Fridovich

    Annu. Rev. Biochem.

    (1975)
  • D.R. Blake et al.

    Lancet

    (1981)
  • J.M. McCord

    Science

    (1974)
  • R.A. Greenwald et al.

    Arthritis Rheum.

    (1980)
  • B. Halliwell et al.
  • Cited by (198)

    • Characterization and functional analysis of peroxiredoxin 4 gene in the Neocaridina denticulata sinensis

      2022, Fish and Shellfish Immunology
      Citation Excerpt :

      According to previous studies, the biological antioxidant system is composed of enzymatic reaction antioxidant system and non-enzymatic reaction antioxidant system. Through their own oxidation, the non-enzymatic antioxidant system can effectively prevent the accumulation of high concentration reactive oxygen species and alleviate the damage of adverse conditions to organisms [15]. The antioxidant system of enzymatic reaction mainly includes catalase, peroxidase, glutathione peroxidase and peroxiredoxin.

    • Characterization of the interaction between carbon black and three important antioxidant proteins using multi spectroscopy and modeling simulations

      2019, Chemosphere
      Citation Excerpt :

      So far most reported works are focused on the toxicity of nanoparticles to cultured cells or animals (Bourdon et al., 2012; Shen et al., 2018), very few on uncovering the deep binding mechanism of nanoparticles with antioxidant proteins. TF, SOD and LYZ are three important antioxidants: TF with available iron-binding sites on it can act as an antioxidant by reducing the concentration of free ferrous iron which can induce oxidative stress by Fenton reaction (Gutteridge, 1986); SOD can catalyze the decomposition of the superoxide radical to hydrogen peroxide and dioxygen (Ward et al., 2018); LYZ is a well-known antibacterial enzyme, but it can also reduce reactive oxygen species (ROS) by eliminating advanced glycation end products (AGE) that can generate ROS (Liu et al., 2006). Increasing evidence confirmed that oxidative stress has a close relationship with the toxicity of nanoparticles and antioxidant enzymes play a key role in keeping the organism from oxidative stress (Fu et al., 2014; Dubey et al., 2015).

    • Protein Antioxidants in Thalassemia

      2013, Advances in Clinical Chemistry
    View all citing articles on Scopus
    View full text