Elsevier

Toxicology

Volume 67, Issue 3, March 1991, Pages 303-323
Toxicology

Studies on lipid peroxidation in rat tissues following administration of low and moderate doses of cadmium chloride

https://doi.org/10.1016/0300-483X(91)90030-5Get rights and content

Abstract

The susceptibility to lipid peroxidation (LPO) of liver, kidneys, brain, lungs, heart, and testes was assessed in rats administered intraperitoneally with various doses of cadmium (Cd). Dose-response studies were carried out with male Long Evans rats (12-week-old; 300 ± 33 g) injected with 25, 125, 500, and 1250 μg Cd/kg as CdCl2 and sacrificed after 24 h. In time-response studies, animals were administered with 25 and 500 μg Cd/kg as CdCl2 and sacrificed after 2, 6, 12, 24, and 72 h. Exposure of rats to low and moderate doses of Cd by the intraperitoneal route stimulated LPO in all the tissues investigated as assessed by the measurement of thiobarbituric acid reactive substances (TBARS). Lungs and brain were the most responsive, and these tissues and liver displayed early responses following Cd exposure. Comparison of LPO to various tissue indicators (for liver: alanine aminotransferase (ALT), sorbitol dehydrogenase (SDH), alkaline phosphatase (ALP); for lungs: ALP, gamma-glutamyl transpeptidase (GGT)) suggested that low doses of Cd stimulated LPO without any evidence of acute damages. These results suggest that LPO is an early and sensitive consequence of Cd exposure as determined in various organs. Investigation of liver, lungs, and heart antioxidant defense system components (glutathione peroxidase (GPX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH), superoxide dismutase (SOD)) revealed that GPX might be considered as a potential modulator of the Cd-induced LPO reaction in lungs and heart tissues.

References (51)

  • T. Ekstrom et al.

    Recovery of malondialdehyde in urine as a 2,4-dinitrophenylhydrazine derivative analyzed with high-performance liquid chromatography

    Chem.-Biol. Interact.

    (1988)
  • H. Ohkawa et al.

    Assay for lipid peroxides in animal tissues by thiobarbituric reaction

    Anal. Biochem.

    (1979)
  • R. Vincent et al.

    A micromethod for the quantitation of cellular protein with the Coomassie brilliant blue dye-binding assay

    Anal. Biochem.

    (1983)
  • N.H. Stacey et al.

    Cadmium toxicity and lipid peroxidation in isolated rat hepatocytes

    Toxicol. Appl. Pharmacol.

    (1980)
  • R.E. Stoll et al.

    Effects of cadmium on nucleic acid and protein synthesis in rat liver

    Toxicol. Appl. Pharmacol.

    (1976)
  • M.M. Jonah et al.

    Early changes in the tissue distribution of cadmium after oral but not intravenous cadmium exposure

    Toxicology

    (1989)
  • K.-L. Wong et al.

    Age difference in the susceptibility to cadmium-induced testicular damages in rats

    Toxicol. Appl. Pharmacol.

    (1980)
  • L. Muller

    Consequences of cadmium toxicity in isolated rat hepatocytes: effects of cadmium on the glutathione-peroxidase system

    Toxicology

    (1986)
  • S. Hirano et al.

    Toxicity of cadmium oxide instilled into the rat lung. II. Inflammatory responses in broncho-alveolar fluid

    Toxicology

    (1989)
  • S. Hirano et al.

    Toxicity of cadmium oxide instilled into the rat lung. I. Metabolism of cadmium oxide in the lung and its effects on essential elements

    Toxicology

    (1989)
  • I.S. Jamall et al.

    Effects of cadmium and dietary selenium on cytoplasmic and mitochondrial antioxidant defense systems in the heart of rats fed high dietary copper

    Toxicol. Appl. Pharmacol.

    (1987)
  • L.J. Marnett et al.

    Prostaglandins

    (1985)
    D.R. Janero et al.

    Cardiac membrane vitamin E and malondialdehyde levels in heart muscle of normotensive and spontaneously-hypertensive rats

    Lipids

    (1989)
  • G.F. Nordberg et al.
  • M. Webb

    The Chemistry, Biochemistry and Biology of Cadmium

    (1979)
  • Z.A. Shaik et al.

    Biological indicators of cadmium exposure and toxicity

    Experientia

    (1984)
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