Water balance effects of systemic and intracerebroventricular administration of angiotensin II in the toad Bufo arenarum

doi:10.1016/0300-9629(89)90002-9

Comparative Biochemistry and Physiology Part A: Physiology

Volume 93, Issue 3, 1989, Pages 505-509

Comparative Biochemi...

https://doi.org/10.1016/0300-9629(89)90002-9 Get rights and content

Abstract

1.
1. Systemic administration of angiotensin II (10 μg/kg) to fully hydrated toads decreased urine production but did not affect natripheric or hydrosmotic water fluxes across the skin.
2.
2. The antidiuretic response was not modified by the simultaneous α-adrenergic blocking with tolazoline (5 mg/kg).
3.
3. In a similar way, single intracerebroventricular injections of 1 μl 10⁻⁴M angiotensin II, decreased urine production but did not affect water uptake.
4.
4. Finally, systemic injections of the angiotensin blocker salarasin (100 μg/kg) did not affect the cutaneous hydrosmotic response of dehydrated toads.
5.
5. We conclude that angiotensin II has no effects on water uptake in this species. The antidiuretic response to angiotensin II, and the involvement of adrenergic mechanisms are discussed.

References (34)

S. Carrick et al.
The renin-angiotens in system and drinking in the euryhaline flounder
Platichthys flesus. Gen. comp. Endocrinol.
(1983)
A. Coviello et al.
Effect of angiotensin II on short circuit current in amphibian membranes
Biochem. Pharmacol.
(1976)
A. Coviello et al.
Role of cyclic AMP and angiotensin II in the response of toad skin to angiotensin II
Biochem. Pharmacol.
(1978)
J. Fernandez-Pardal et al.
Angiotensin converting enzyme in the toad Bufo arenarum
Comp. Biochem. Physiol.
(1986)
Y. Hasegawa et al.
Chemical structure of angiotensin in the bullfrog Rana catesbeiana
Gen comp. Endocrinol.
(1983)
H. Kobayashi et al.
Drinking induced by angiotensin II in fishes
Gen comp. Endocrinol.
(1983)
H. Nolly et al.
The renin-angiotensin system in Bufo arenarum and Bufo paracnemis
Comp. Biochem. Physiol.
(1971)
M.O. Soria et al.
Comparative effects of angiotensin II on osmotic water permeability in the toad (Bufo arenarum)
Combp. Biochem. Physiol.
(1987)
Y. Takei
Angiotensin and water intake in the Japanese quail (Coturnix coturnix japonica)
Gen comp. Endocrinol.
(1977)
Y. Takei et al.
Angiotensin and water intake in the Japanese eel, Anguilla japonica
Gen comp. Endocrinol.
(1979)

M. Wada et al.

Induction of drinking in the white-crowned sparrow, Zonotrichia leucophris gambelii, by intracranial injection of angiotensin II

Gen comp. Endocr.

(1975)

D. Beasley et al.

Angiotensin-stimulated drinking in marine fish

Am. J. Physiol.

(1986)

P.J. Bentley et al.

Do frogs drink?

J. exp. Biol.

(1979)

R.G. Carroll et al.

Evolution of angiotensin II-mduced catecholamine release

Am. J. Physiol.

(1982)

A. Coviello et al.

Hydrosmotic effect of angiotensin II in isolated toad skin

Acta physiol. latinoam.

(1973)

A.N. Epstein et al.

Angiotensin as a dipsogen

A.N. Epstein et al.

Drinking induced by injection of angiotensin into the brain of the rat

J. Physiol.

(1970)

Cited by (13)

Comparison of ACE activity in amphibian tissues: Rana esculenta and Xenopus laevis
2007, Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Angiotensin converting enzyme (ACE) is the dipeptidyl-carboxypeptidase of the renin–angiotensin system involved in the control of blood pressure and hydromineral metabolism. It converts angiotensin I to angiotensin II, the biologically active octapeptide. Angiotensin converting enzyme-like activity has been demonstrated in a wide range of vertebrates. The presence of ACE was investigated in tissues of two amphibian species, the frog Rana esculenta and the toad Xenopus laevis. ACE activities were determined by specific substrate hydrolysis in gut, gonads, lung, kidney, heart, liver, skin, erythrocytes, and muscle homogenates and plasma by means of high performance liquid chromatography. Significant ACE activity was found in gut, gonads, lung and kidney, while that in heart, liver, skin, erythrocytes, muscle, and plasma was very low. Testis of toad contained the highest ACE activity, while that in erythrocytes of male and female frogs was notable.
Comparative analysis of amphibian and mammalian angiotensin receptors
2001, Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Amphibian angiotensin receptors (xAT receptors) share many similarities with mammalian type 1 angiotensin receptors (AT₁ receptors). Both xAT and AT₁ receptors belong to the super family of seven transmembrane spanning G protein-coupled receptors and share approximately 60% amino acid homology. Highly stable secondary structure in the 5′ leader sequences and the presence of the mRNA destabilizing sequence (AUUUA) in the 3′ untranslated region (3′UTR) of the xAT and AT₁ receptor mRNAs suggest similar mechanisms exist for regulating gene expression. Amphibian and mammalian AT receptors bind angiotensin with equivalent affinities but show marked differences in their affinities towards mammalian AT₁ receptor subtype selective non-peptide ligands. Both xAT and AT₁ receptors couple to G proteins and to the phospholipase C (PLC) signal transduction pathway. Mammalian AT₁ receptors play a key role in maintaining blood pressure and fluid homeostasis and there is considerable evidence that xAT receptors play a similarly important role in amphibians. This review focuses on the comparison of amphibian xAT receptors with mammalian AT₁ receptors in terms of their structure, pharmacology, signaling, and function.
Angiotensin II elicits water seeking behavior and the water absorption response in the toad Bufo bufo
2001, Hormones and Behavior
Fully hydrated toads, Bufo bufo were acclimated to a simulated terrestrial habitat, with access to shelters and water. To get from the shelters to the water, the toads had to walk across the pan of an Ohaus balance and the body weights were recorded on a computer. Toads were placed inside shelters immediately following injection of human angiotensin II (A II), Thr⁸-saralasin, or Ringer's in the dorsal lymph sac, and their behavior was recorded continuously by video surveillance. The injection doses were 1–100 μg/100 g body weight A II and 100 μg/100 g body weight saralasin dissolved in 0.1 ml Ringer's; control animals received the same volume of Ringer's. The latency from injection to the initiation of water absorption behavior (WR) was significantly shorter in both A-II- and saralasin-injected toads, compared to controls. A-II- and saralasin-injected toads also spent significantly more time in the water than controls. The bladder depots when WR was terminated were significantly larger in A-II- or saralasin-injected toads than in controls. The stimulatory action of Thr⁸-saralasin, an antagonist of A II in mammals, on WR behavior in B. bufo suggests differences in receptor structure and/or receptor distribution between amphibians and mammals.
Interaction between antagonists of angiotensin II and antidiuretic hormone in isolated toad tissues
1996, Comparative Biochemistry and Physiology - A Physiology
Responses of isolated aorta and toad skin from Bufo arenarum to angiotensin II (AT II) and antidiuretic hormone (ADH) were examined. Inhibitory effects on both responses were obtained either by AT II antagonist or ADH (ADHant). Contractile responses to AT II and AVT were inhibited in a similar way by both Leu⁸ AT II and ADHant. No blocking effect could be obtained against norepinephrine. Leu⁸ AT II, ADHant and an oxytocin antagonist were able to inhibit osmotic water permeability (P_osm) and short-circuit current (SCC) response in toad skin. The inhibitor not only blocked its own agonist response but also other peptide agonistics' responses. No antagonist affected P_osm response to isoproterenol (Isop). The striking similarities among ADH and AT II receptors in amphibian tissues suggest a common peptide hormone receptor.
Localization and quantification of angiotensin II (A II) binding sites in the kidney of Xenopus laevis-Lack of A II receptors in the adrenal tissue
1992, General and Comparative Endocrinology
The distribution and properties of angiotensin binding sites in the kidney of the clawed toad Xenopus laevis were studied using quantitative in vitro autoradiography. Specific binding sites for [¹²⁵I]-[Val⁵]-angiotensin II (A II) were located in the glomeruli of the kidney but not in the adrenal tissue. [¹²⁵I]-[Val⁵]-A II binding was equilibrated after 45 min. Scatchard and Hill analyses of saturation experiments showed that [¹²⁵I]-[Val⁵]-A II binds to a single class of binding sites with a dissociation constant (K_d) of 1.884 ± 0.535 nM and a maximum binding capacity (B_max) of 0.484 ± 0.144 fmol/mm² (n = 8). Various angiotensin analogues displaced [¹²⁵I]-[Val⁵]-A II in the rank order [Sar¹, Ile⁵]-A II > human A II > [¹²⁵I]-[Val⁵]-A II = [Val⁵]-A II = human A III ⪢ human A I. Unrelated peptides did not alter the binding of [¹²⁵I]-[Val⁵]-A II. Acclimation to 1.5% seawater increased [¹²⁵I]-[Val⁵]-A II binding in glomeruli after 12 hr but returned to control levels after 7 days. Steroidogenic and catecholaminergic actions of [Val⁵]-A II on the adrenal tissue were examined in vitro and in vivo. Compared with known interrenal stimulators [human ACTH(1–39) and AVT] minimal effects were obtained only in vitro with high doses of [Val⁵]-A II while catecholamine release was unaffected. In vivo a single injection of 3 nmol [Val⁵]-A II per 100 g body wt did not change serum levels of corticosterone, aldosterone, epinephrine, norepinephrine, or dopamine.
Angiotensin II binding sites in frog kidney and adrenal
1992, Peptides
Angiotensin II (AII) binding sites were localized and quantified in kidney and adrenal of the frog Rana temporaria by quantitative in vitro autoradiography. AII binding was present in kidney glomeruli and in interrenal tissue of the outer zone of the adrenal gland. Saturation experiments showed that [¹²⁵I]-[Val⁵]AII binds toa single class of binding sites with a dissociation constant (K_d) of 548 ± 125 pM in glomeruli and 593 ± 185 pM in interrenal tissue (n = 8). The corresponding maximal binding capacities (B_max) were 2.48 ± 0.71 and 3.05 ± 1.02 fmol/mm², respectively. AII binding was displaced by unlabeled angiotensin analogues in the rank order: $[Sar^{1}]AII > human AII > [^{125} I]-[Val^{5}]AII = [Val^{5}]AII = human AIII > > human AI$ . The AII binding sites in glomeruli and interrenal tissue suggest an influence of AII on glomerular filtration rate and adrenal steroid secretion to take part in osmomineral regulation of the frog.

View all citing articles on Scopus

View full text

Water balance effects of systemic and intracerebroventricular administration of angiotensin II in the toad Bufo arenarum

Abstract

Platichthys flesus. Gen. comp. Endocrinol.

Biochem. Pharmacol.

Biochem. Pharmacol.

Comp. Biochem. Physiol.

Gen comp. Endocrinol.

Gen comp. Endocrinol.

Comp. Biochem. Physiol.

Combp. Biochem. Physiol.

Gen comp. Endocrinol.

Gen comp. Endocrinol.

Gen comp. Endocr.

Angiotensin-stimulated drinking in marine fish

Am. J. Physiol.

Do frogs drink?

J. exp. Biol.

Evolution of angiotensin II-mduced catecholamine release

Am. J. Physiol.

Hydrosmotic effect of angiotensin II in isolated toad skin

Acta physiol. latinoam.

Angiotensin as a dipsogen

Drinking induced by injection of angiotensin into the brain of the rat

J. Physiol.