Cancer Letters

Cancer Letters

Volume 107, Issue 2, 22 October 1996, Pages 229-233
Cancer Letters

A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies

https://doi.org/10.1016/0304-3835(96)04832-XGet rights and content

Abstract

A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost required to carry out such analysis. It will also significantly enhance the ability to use genetic screening techniques as a potential tool for early detection of health outcomes in exposed populations.

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    CYP2C9∗3 (rs1057910), NAT2∗5 (rs1801280), NAT2∗6 (rs1799930), NAT2∗7 (rs1799931), GSTP1 (rs1695) and GCLC (rs761142) alleles were genotyped by TaqMan Drug Metabolism Genotyping Assays (Applied Biosystems, Foster City, California, USA on a QuantStudio™ 6 Flex Machine). While genotyping of GSTT1 and GSTM1 polymorphisms was performed by multiplex polymerase chain reactions as previously described [23,24]. The allele frequencies and genotype frequencies of the HLA class I, CYP2C9∗3, NAT2∗5, NAT2∗6, NAT2∗7, GSTP1 (rs1695), GSTT1 (null), GSTM1 (null) and GCLC (rs761142), were determined by direct counting.

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