Central projections of primary afferent fibers from the rat trigeminal nerve labeled with isolectin B4-HRP
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Cited by (18)
A strategy for bypassing the blood-brain barrier: Facial intradermal brain-targeted delivery via the trigeminal nerve
2017, Journal of Controlled ReleaseCitation Excerpt :Facial i.d. injection might deliver drugs to the human brain via the trigeminal nerve. Accumulating evidences indicates that facial intradermal injection might deliver lectins and HRP to the trigeminal nerve [13–16]. However, currently, facial intradermal injection of drugs to treat human CNS diseases has not been reported.
Gabaergic influence on temporomandibular joint-responsive spinomedullary neurons depends on estrogen status
2014, NeuroscienceCitation Excerpt :The TMJ region is supplied by small-diameter sensory fibers (Kido et al., 1995; Takeuchi and Toda, 2003; Ioi et al., 2006) that project to the superficial laminae at the Vc/C1–2 region (Shigenaga et al., 1986, 1988). The superficial laminae at the Vc/C1–2 region share many properties with corresponding regions at lower segments of the spinal cord (Bereiter et al., 2000) and receive the majority of input from unmyelinated sensory fibers (Kobayashi and Matsumura, 1996; Sugimoto et al., 1997). Mechanisms of central sensitization and disinhibition of inhibitory signals within the superficial laminae are thought to be critical for the development of chronic pain (see Woolf and Salter, 2000; Suzuki et al., 2002; Todd, 2010).
Estrogen status and psychophysical stress modify temporomandibular joint input to medullary dorsal horn neurons in a lamina-specific manner in female rats
2013, PainCitation Excerpt :A similar laminar pattern is seen for TMJ and jaw muscles afferents at the Vc/C1–2 region [5,21,63]. The TMJ and jaw muscles are supplied mainly by small diameter sensory fibers [40,68] that express TRPV1 [18,35] or isolectin B4 (IB4) [3], whereas the expression of TRPV1 [6] and IB4 [41,65] at the Vc/C1–2 region is restricted to the superficial laminae. Thus, a peripheral basis for estrogen status and stress induced lamina-specificity in medullary dorsal horn could derive from preferential actions on unmyelinated C fibers.
Distinct time courses of microglial and astrocytic hyperactivation and the glial contribution to pain hypersensitivity in a facial cancer model
2012, Brain ResearchCitation Excerpt :Forty micrometer-thick serial sections were cut using a freezing microtome (WS-112-N, Yamatokohki, Saitama, Japan). An earlier study reported that the vibrissal pad- and infraorbital area-innervated regions in the trigeminocervical complex are located 2–3 and 4–5 mm caudal to the obex, respectively (Kobayashi and Matsumura, 1996); therefore, the sections from these two respective regions were taken to be the MDH and UCDH. The free-floating sections were incubated in 3% normal goat serum in phosphate-buffered saline for 1 h at room temperature and then incubated with anti-Iba1 rabbit polyclonal antibody (diluted 1:300, 019-19741, Wako) or anti-GFAP antibody (diluted 1:200) overnight at 4 °C.
Unmyelinated fibers of the anterior ethmoidal nerve in the rat co-localize with neurons in the medullary dorsal horn and ventrolateral medulla activated by nasal stimulation
2009, Brain ResearchCitation Excerpt :Presumably this is because WGA has high affinity for N-acetyl-d-glucosamine, a carbohydrate found on the plasma membrane of small diameter afferent fibers (Nagata and Burger, 1974). In contrast, HRP conjugated with Isolectin-B4 from Bandeiraea simplicifolia (IB4-HRP) has been used to selectively trace unmyelinated C-fibers (Kitchener et al., 1993; Kobayashi and Matsumura, 1996; Wang et al., 1998). IB4 binds to terminal α-d-galactose groups of the plasma membrane of nervous tissue (Ambalavanar and Morris, 1992; Ambalavanar and Morris, 1993), and so IB4-HRP labels only unmyelinated neurons because the myelin sheathes of myelinated axons do not contain galactose glycoconjugates (Streit et al., 1985).