Clinical original contribution
Radiation-induced apoptosis: Relevance to radiotherapy

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Abstract

Radiation-induced apoptosis is reviewed in terms of: (a) the identification of apoptotic and necrotic cells, (b) observations in vitro and in vivo of radiation-induced apoptosis, (c) genes controlling apoptosis, (d) evidence that the target may be the plasma membrane or nuclear DNA, (e) quantitative comparisons of apoptotic death and reproductive (clonogenic) death, (f) the importance of radiation-induced apoptosis in radiotherapy, and (g) studies of radiation-induced apoptosis that are needed. High priority should be placed on determining the molecular pathways that are important in the expression and modulation of radiation-induced apoptosis. Specifically, the events that modulate the apoptosis that occurs in interphase before the cell can divide should be distinguished from the events before division that modulate the misrepair of DNA damage, that results in chromosomal aberrations observed in mitotic cells, which in turn cause the progeny of the dividing cell with aberrations to die by either apoptosis or necrosis. Then, molecular events that determine whether a cell that divides with or without a chromosomal aberration will produce progeny that apoptose or necrose need to be identified. These considerations are important for determining how modulation of radiation-induced apoptosis will affect the ultimate clonogenic survival, and possibly genomic instability in the surviving progeny.

References (78)

  • D.J. Allan

    Radiation-induced apoptosis: Its role in a MADCaT (mitosis-apoptosis-differentiation-calcium toxicity) scheme of cytotoxicity mechanisms

    Int. J. Radiat. Biol.

    (1992)
  • P.J. Barr et al.

    Apoptosis and its role in human disease

    Biotechnology

    (1994)
  • N.A. Berger et al.

    Role of nicotinamide adenine dinucleotide and adenoside triphosphate in glucocorticoid-induced cytotoxicity in susceptible lymphoid cells

    J. Clin. Invest.

    (1987)
  • C.H. Chen et al.

    Transfected c-myc and c-Ha-ras modulate radiation-induced apoptosis in rat embryo cells

    Radiat. Res.

    (1994)
  • M. Chen et al.

    Suppression of Bcl-2 messenger RNA production may mediate apoptosis after ionizing radiation, tumor necrosis factor alpha, and ceramide

    Cancer Res.

    (1995)
  • F.J. Chrest et al.

    Identification and quantitation of apoptotic cells following anti-CD3 activation of murine Go T-cells

    Cytometry

    (1993)
  • M.N. Cornforth et al.

    A quantitative comparison of potentially lethal damage repair and the rejoining of interphase chromosome breaks in low passage normal human fibroblasts

    Radiat. Res.

    (1987)
  • R.G. Dale

    The application of the linear-quadratic dose-effect equation to fractionated and protracted radiotherapy

    Br. J. Radiol.

    (1985)
  • M.F. Denisenko et al.

    Is the NAD-poly(ADP-ribose)polymerase system the trigger in radiation-induced death of mouse thymocytes?

    Int. J. Radiat. Biol.

    (1989)
  • W.C. Dewey et al.

    Chromosomal aberrations and mortality of x-irradiated mammalian cells: Emphasis on repair

  • A. Di Leonardo et al.

    DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts

    Genes Dev.

    (1994)
  • M.M. Elkind et al.

    The radiobiology of cultured mammalian cells

  • H.H. Evans et al.

    DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines

    Radiat. Res.

    (1993)
  • T.M. Fliedner et al.

    Structural, cytological and radiographic (H3-thymidine) changes in the bone marrow following total body irradiation

    Am. J. Pathol.

    (1961)
  • J.F. Fowler

    Review: The linear-quadratic formula and progress in fractionated radiotherapy

    Br. J. Radiol.

    (1989)
  • P. Fritsch et al.

    Kinetics of radiation-induced apoptosis in the cerebellem of 14-day-old rats after acute or during continuous exposure

    Int. J. Radiat. Biol.

    (1994)
  • Z. Fuks et al.

    Basic fibroblast growth factor protects endothelial cells against radiation-induced programmed cell death in vitro and in vivo

    Cancer Res.

    (1994)
  • W. Gorczyca et al.

    Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA

    Cytometry

    (1994)
  • D.A. Haas-Kogan et al.

    Inhibition of apoptosis by the retinoblastoma gene product

    EMBO J.

    (1995)
  • A. Haimovitz-Friedman et al.

    Protein kinase C mediates basic fibroblast growth factor protection of endothelial cells against radiation-induced apoptosis

    Cancer Res.

    (1994)
  • A. Haimovitz-Friedman et al.

    Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis

    J. Exp. Med.

    (1994)
  • E.J. Hall et al.

    Radiation response characteristics of human cells in vitro

    Radiat. Res.

    (1988)
  • S. Hellman

    Principles of radiation therapy

  • J.H. Hendry et al.

    Intestinal cell radiosensitivity: A comparison for cell death assayed by apoptosis or by a loss of clonogenicity

    Int. J. Radiat. Biol.

    (1982)
  • J.H. Hendry et al.

    Cell death (apoptosis) in the mouse small intestine after low doses: Effects of dose-rate, 14.7 MeV neutrons, and 600 MeV (maximum energy) neutrons

    Int. J. Radiat. Biol.

    (1982)
  • S.H. Kaufmann et al.

    Specific proteolytic cleavage of poly-(ADP-ribose)polymerase: An early marker of chemotherapy-induced apoptosis

    Cancer Res.

    (1993)
  • J.F.R. Kerr et al.

    Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics

    Br. J. Cancer

    (1972)
  • N.V. Klassen et al.

    Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymocytes

    Int. J. Radiat. Biol.

    (1993)
  • Y.A. Lazebnik et al.

    Nuclear events of apoptosis in vitro in cell-free mitotic extracts: A model system for analysis of the active phase of apoptosis

    J. Cell Biol.

    (1993)
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