Elsevier

Gene

Volume 174, Issue 1, 26 September 1996, Pages 129-134
Gene

Molecular cloning of two novel rab genes from human melanocytes

https://doi.org/10.1016/0378-1119(96)00509-4Get rights and content

Abstract

We isolated the genes of two small GTP-binding proteins of the rab family from a human melanocyte cDNA library and from melanoma cells. One gene, rab30 codes for a novel rab protein of 203 amino acids with minimal homology to previously documented GTPases. The other, rab22b, appears to be an isoform of the human homologue of canine rab22. Both rab mRNAs displayed a nearly ubiquitous pattern of expression in the various tissues examined. Rab22b and rab30 were mapped to chromosomes 18 and 11, respectively.

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    RAB30 was originally discovered from cDNA extracted from human melanocytes and was mapped to chromosome 11 [1,2]. The RAB30 sequence contains the generic isoprenylation sequence (CCAAX) allowing for physical attachment to the membrane bilayer through a geranylgeranyl lipid anchor [1]. Rab30 has been shown to be required for embryo development in Drosophila melanogaster [3], although the function of this small G protein still remains unclear.

  • JNK signalling influences intracellular trafficking during Drosophila morphogenesis through regulation of the novel target gene Rab30

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