Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte)

doi:10.1016/0378-1135(83)90076-7

Veterinary Microbiology

Volume 8, Issue 3, June 1983, Pages 237-249

https://doi.org/10.1016/0378-1135(83)90076-7 Get rights and content

Abstract

The infective agent of jaagsiekte was shown to be present in the fluid which accumulates in the respiratory tract of sheep during the terminal stages of the disease. The fluid also contained reverse transcriptase (RT) activity which showed a clear preference for a ribonucleic acid synthetic template over the corresponding deoxyribonucleic acid template and which utilised the RT specific template/primer poly (2′-0-methylcytidylate) oligodeoxyguanylate. This enzyme activity was associated with a particle which had typical retroviral buoyant densities in a range of gradient media.

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Cited by (30)

Sheep pulmonary adenomatosis: Characterization of two pathological forms associated with jaagsiekte retrovirus
2000, Journal of Comparative Pathology
Pathological and immunohistochemical studies were performed on the lungs of 10 sheep with lesions of “classical” sheep pulmonary adenomatosis (SPA) and six sheep with “atypical” lung tumours. Lung tumour samples and other tissues from the same 16 animals were tested for the presence of jaagsiekte retrovirus (JSRV) by a polymerase chain reaction (PCR) that amplified a portion of the U3 long terminal repeat. The differences in the gross appearance of the classical and atypical forms paralleled the histopathological differences. The latter mainly concerned the stroma of the tumours which in the atypical cases was more heavily infiltrated by inflammatory cells and connective tissue fibres. JSRV major capsid protein was detected immunohistochemically in the epithelial transformed cells of both classical and atypical tumours, but the immune reactivity was slightly milder in atypical SPA. Proviral U3 sequences of JSRV were detected by specific PCR in all the tumour samples. Furthermore, the sequences of amplimers obtained from the two different pathological forms of the tumour were very similar. However, the dissemination of JSRV to other organs was greater in sheep with classical SPA than in those with atypical SPA. The pathological and virological features of these two forms of tumour are compared in an attempt to clarify whether classical and atypical SPA are two separate diseases or different expressions of a single disease spectrum.
Retroviral aetiopathogenesis of ovine pulmonary carcinoma: A critical appraisal
1996, British Veterinary Journal
Although it has long been thought that a retrovirus is the responsible agent for ovine pulmonary carcinoma (OPC), identification of a replicative viral agent has proven difficult. Recently, the genome of a new retrovirus, jaagsiekte sheep retrovirus (JSRV), found in the lung-was of affected sheep lung, has bee cloned and sequenced; characterization of this virus and its consistent presence in tumor cells argue for its role as the aetiologic agent of OPC. Analysis of the nucleic acid sequence of the JRSV genome, suggests a new class of retrovirus, one that is chimeric according to the morphological classification scheme used for retroviruses. The genome of this virus does not appear to contain an oncogene, and the mechanism by which it causes disease is still unknown. The presence of multiple copies of endogenous retroviruses related to JSRV in DNA of OPC-affected and unaffected sheep further complicates investigation of oncogenesis in OPC. This review examines the evidence for a retrovirus as the causative agent for OPC, with particular emphasis on the viruses studied to date. The significance of endogenous, JSRV-related sequences is considered. The mechanisms by which a retrovirus such as JSRV might induce lung tumours in sheep, and which of these are most likely, are discussed in light of these developments, as are the prospects for new means of diagnosis and treatment of this disease.
Development and application of an antibody ELISA for the marker protein of ovine pulmonary carcinoma
1995, Veterinary Immunology and Immunopathology
Ovine pulmonary carcinoma (OPC) is a contagious pulmonary neoplasia with a suspected retroviral etiology. The major core protein (P27) of the putative OPC virus cross-reacts with antibodies to P27 of the Mason-Pfizer monkey virus (MPMV), a type-D retrovirus. This serological reactivity serves as the only accepted biological marker for OPC. In order to make a useful reagent for the detection of the OPC marker for serodiagnosis and epidemiological studies, the MPMV-P27 coding region was cloned and expressed in Escherichia coli. Gel purified recombinant MPMV-P27 protein was used to develop an immunoassay. This recombinant enzyme-linked immunosorbent assay (ELISA) was then used to screen 223 sera from US sheep and 176 sera from Italian sheep. In this study, we found: (1) a high prevalence of infection with the putative OPC retrovirus in sheep with chronic pneumonia; (2) a subclinical infection with OPC virus may be more common in US sheep than indicated by the rare recorded occurrence of pulmonary carcinoma; (3) an apparent association between ovine lentivirus (OLV) and OPC infection.
Evidence for retroviral capsid and nucleocapsid antigens in ovine pulmonary carcinoma
1992, Virus Research
A retroviral etiology has been proposed for ovine pulmonary carcinoma (OPC); however, the putative virus (OPCV) has yet to be cultured. A Western immunoblotting assay using a panel of retroviral antisera was developed to further define the structural proteins of the virus associated with OPC and to confirm their presence in tumor samples of affected sheep. The results confirmed that the main structural viral component, the capsid protein (CA), was present in tumor materials (lung fluids, lavages, and tumor homogenates) but not in similar samples from control subjects. A second viral protein was detected in the tumor samples by antisera to the nucleocapsid protein (NC) of type D retroviruses. Both components could be purified from the tumor material in a manner consistent with association in viral particles. The cross-reactivity of the OPC antigens to other type B and D retroviruses was assessed. These results suggest that OPC antigens are closely related to the structural proteins of several type D primate retroviruses.
Molecular mechanisms in lung pathogenesis
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Maedi-visna and sheep pulmonary adenomatosis: A study of concurrent infection
1990, British Veterinary Journal
The transmission of maedi-visna (MV) virus was studied within two groups of sheep, one of which was also affected with sheep pulmonary adenomatosis (SPA). Serological monitoring of sheep kept in contact with both groups indicated that MV virus replication occurred to a greater extent in the group with both diseases, three of five in-contact sheep being seropositive after 1 year's exposure compared with none of six held with MV-virus-only infected lambs.

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Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte)

Abstract

Virology

Virology

Virology

Comp. Immun. Microbiol. Infect. Dis.

FEBS Lett.

Virology

Virology

Biochem. Biophys. Acta

L'adenomatosi pulmonare della pecora (jaagsiekte) studio ultrastructurale

Lav. Ist. Anat. Istol. Patol. Univ. Studi Perugia

The demonstration of maedi-visna virus in sheep in Great Britain

Vet. Rec.

Experiments with jaagsiekte

Am. J. Pathol.

Type D primate retroviruses: a review

Cancer Res.

Poly(2′-0-methylcytidylate) oligodeoxyguanylate as a template for the RNA directed DNA polymerase in RNA tumour virus particles and a specific probe for the RNA directed enzyme in transformed murine cells

Biochemistry