Elsevier

Genomics

Volume 25, Issue 1, 1 January 1995, Pages 66-72
Genomics

Genetic mapping in human and mouse of the locus encoding TRBP, a protein that binds the TAR region of the human immunodeficiency virus (HIV-1)

https://doi.org/10.1016/0888-7543(95)80110-8Get rights and content

Abstract

Productive infection with HIV-1, the virus responsible for AIDS, requires the involvement of host cell factors for completion of the replicative cycle, but the identification of these factors and elucidation of their specific functions has been difficult. A human cDNA, TRBP, was recently cloned and characterized as a positive regulator of gene expression that binds to the TAR region of the HIV-1 genome. Here we demonstrate that this factor is encoded by a gene, TARBP2, that maps to human chromosome 12 and mouse chromosome 15, and we also identify and map one human pseudogene (TARBP2P) and two mouse TRBP-related sequences (Tarbp2-rs1, Tarbp2-rs2). The map location of the expressed gene identifies it as a candidate for the previously identified factor encoded on human chromosome 12 that has been shown to be important for expression of HIV-1 genes. Western blotting indicates that despite high sequence conservation in human and mouse, the TARBP2 protein differs in apparent size in primate and rodent cells.

References (41)

  • M.C. Adamson et al.

    The mouse homolog of the Gibbon ape leukemia virus receptor: Genetic mapping and a possible function in rodents

    Virology

    (1991)
  • A. Alonso et al.

    Human chromosome 12 is required for optimal interactions between TAT and TAR of human immunodeficiency virus type 1 in rodent cells

    J. Virol.

    (1992)
  • R.J. Bandziulius et al.

    RNA-binding proteins as developmental regulators

    Genes Dev.

    (1989)
  • F. Barre-Sinoussi et al.

    Isolation of a T-lymphocyte retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS)

    Science

    (1983)
  • B. Berkhout et al.

    Trans-activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: A quantitative analysis

    J. Virol.

    (1989)
  • B. Berkhout et al.

    Detailed mutational analysis of TAR RNA: Critical spacing between the bulge and loop recognition domains

    Nucleic Acids Res.

    (1991)
  • R.C. Gallo et al.

    Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk of AIDS

    Science

    (1984)
  • A. Gatignol et al.

    Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR

    Science

    (1991)
  • A. Gatignol et al.

    Relatedness of an RNA binding motif in HIV-1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila Staufen

    Mol. Cell. Biol.

    (1993)
  • A. Gatignol et al.

    Expression cloning of genes encoding RNA-binding proteins

    Methods Mol. Genet.

    (1994)
  • Cited by (33)

    • The distribution of Neuropeptide FF and Neuropeptide VF in central and peripheral tissues and their role in energy homeostasis control

      2021, Neuropeptides
      Citation Excerpt :

      The NPFF and NPAF peptides are encoded by a single Npff gene that was first cloned in human (Perry et al., 1997) and subsequently in bovine, rat and mouse (Vilim et al., 1999). The human Npff gene is localized on the long arm of chromosome 12 (12q13) (Schulz et al., 2002), while mice have their Npff gene on chromosome 15 (57.65 cM, cytoband F3) (Kozak et al., 1995; Nystedt et al., 2006). The organization of the Npff gene is conserved in all four species with three exons and two introns, both of the latter falling into coding regions (Perry et al., 1997; Vilim et al., 1999) (Fig. 1).

    • The merlin interacting proteins reveal multiple targets for NF2 therapy

      2008, Biochimica et Biophysica Acta - Reviews on Cancer
    View all citing articles on Scopus
    View full text