Angiotensin II inhibits calcium and M current channels in rat sympathetic neurons via G proteins

doi:10.1016/0896-6273(94)90447-2

Neuron

Volume 12, Issue 6, June 1994, Pages 1319-1329

https://doi.org/10.1016/0896-6273(94)90447-2 Get rights and content

Abstract

We characterized inhibition of N-type Ca²⁺ and M current K⁺ channels in rat superior cervical ganglion neurons by angiotensin II (angioII) using the patch clamp. Of 120 neurons, 97 showed inhibition of I_Ca (mean 32%), which was slow in onset and very slow to reverse under whole-cell recording conditions. This inhibition was blocked by the AT₁ receptor antagonist losartan, attenuated by inclusion of 2 mM GDP-β-S in the pipette, mostly pertussis toxin insensitive, half-sensitive to N-ethylmaleimide, and wholly voltage independent. With 20 mM instead of 0.1 mM BAPTA in the pipette, the inhibition was strongly attenuated; however, we detected no angioII-induced [Ca²⁺]_i signal using the fluorescent indicator indo-1. I_Ba from cell-attached patches was reduced by bath-applied angioll (mean 33%), suggesting use of a diffusible cytoplasmic messenger. M currents were inhibited by angioll in 8 of 11 neurons (mean 50%) cultured overnight. Hence, a second agonist, angioll, may share the slow, second messenger-utilizing, pertussis toxin-insensitive signaling pathway used by muscarinic agonists.

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The channel is encoded by the Kv7/KCNQ potassium family, with heteromers composed most often of the Kv7.2 and Kv7.3 subunits, although other Kv7 members may also contribute (Wang et al., 1998; Passmore et al., 2003; Miceli et al., 2008). The Kv7/M current can be modulated by many neuronal transmitters and peptides, and great achievements have been made toward understanding the underlying molecular mechanism(s) (Shapiro et al., 1994; Cruzblanca et al., 1998; Wallace et al., 2002; Gamper and Shapiro, 2003; Zhang et al., 2011). The most well-studied regulation of Kv7/M is mediated through Gq protein-coupled membrane receptors (GqPCRs) involving two key signaling molecules, phosphatidylinositol 4,5-bisphosphates (PIP2) and intracellular Ca2+ (Yu et al., 1994; Brown et al., 2007; Hughes et al., 2007).
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^☆: The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordancewith 18 USC Section 1734 solely to indicate this fact.

^∗: Present address: Abteilung Zellphysiologie, Max-Planck-Institut für medizinische Forschung, lahnstrasse 29, D-69120 Heidelberg, Federal Republic of Germany.

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Neuron

ArticleAngiotensin II inhibits calcium and M current channels in rat sympathetic neurons via G proteins☆

Abstract

Brain Res. Rev.

Neuron

Neuron

Front. Neuroendocrinol.

Brain Res.

FEBS Lett.

Brain Res.

Neurosci. Lett.

Neuron

Biochem. Biophys. Res. Commun.

Brain Res.

Neuron

J. Biol. Chem.

FEBS Lett.

Neuron

Neuron

Neuron

Neuron

Neuron

Neuron

Brain Res.

Eur. J. Pharmacol.

Luteinizing hormonereleasing factor and muscarinic agonists act on the same voltagesensitive K+ current in bullfrog sympathetic neurones

Br. J. Pharmacol.

Charge movement associated with the opening and closing of the activation gates of the Na channels

J. Gen. Physiol.

Neurotransmitter inhibition of neuronal calcium channels by changes in channel voltage dependence

Nature

Intracellular Cal' buffers disrupt muscarinic suppression of Ca2+ current and M current in rat sympathetic neurons

Modulation of N-type calcium channels in bullfrog sympathetic neurons by luteinizing hormone-releasing hormone: kinetics and voltage dependence

J. Neurosci.

ω-Conotoxin block of N-type calcium channels in frog and rat sympathetic neurons

J. Neurosci.

Control of glomerulosa cell function by angiotensin II: transduction by G proteins and inositol polyphosphates

Clin. Exp. Pharmacol. Physiol.

Article
Angiotensin II inhibits calcium and M current channels in rat sympathetic neurons via G proteins☆

Luteinizing hormonereleasing factor and muscarinic agonists act on the same voltagesensitive K⁺ current in bullfrog sympathetic neurones

Intracellular Cal' buffers disrupt muscarinic suppression of Ca²⁺ current and M current in rat sympathetic neurons