Regular paper
Role of different subtypes of P2 purinoceptor on cytosolic Ca2+ levels in rat aortic smooth muscle

https://doi.org/10.1016/0922-4106(94)90135-XGet rights and content

Abstract

The role of different subtypes of P2 purinoceptors on cytosolic Ca2+ level ([Ca2+]i) was examined in vascular smooth muscle of rat aorta. αβ-Methylene-ATP (P2x agonist), 2-methylthio-ATP (P2Y agonist), UTP and ATPγS (P2U agonists), and ATP (nonselective P2 agonist) induced a transient increase followed by a small sustained increase in [Ca2+]i in a concentration dependent manner. Among these agonists, αβ-methylene-ATP was the most potent. In the absence of extracellular Ca2+ (with 0.5 mM EGTA), ATP, UTP and ATPγS induced a transient increase in [Ca2+]i whereas αβ-methylene-ATP and 2-methylthio-ATP and ATPγS induced a rapid increase in [Ca2+]i followed by a sustained increase while αβ-methylene-ATP and 2-methylthio-ATP were ineffective. These results suggest that Ca2+ release from the intracellular Ca2+ store is mediated by P2U purinoceptor whereas Ca2+ influx is mediated by both P2X and P2U purinoceptors in the rat aortic smooth muscle.

References (21)

There are more references available in the full text version of this article.

Cited by (0)

View full text