Elsevier

Clinical Biochemistry

Volume 18, Issue 4, August 1985, Pages 220-223
Clinical Biochemistry

Determination of prolinase activity in plasma. Application to liver disease and its relation with prolidase activity1

https://doi.org/10.1016/S0009-9120(85)80043-6Get rights and content

We describe prolinase (EC 3.4.13.8) activity in human plasma for the first time. Optimum activity was obtained with prolyvaline as substrate and 0.02 mmol/L manganese concentration at pH 9.0. Moreover, preincubation with manganese was not required, contrary to prolidase (EC 3.4.13.9) activity. The mean value observed in 106 subjects without liver and renal disorders was 16 U/L ± 14 (2 SD). We determined this plasma enzyme activity in patients with acute hepatitis and chronic liver disease. Plasma prolinase activity was strongly dependent upon cytolysis because of the high activity in liver and the low activity in plasma. Of 24 patients with chronic liver disease (4 chronic hepatitis and 20 cirrhosis) and without cytolysis, prolinase activity was slightly increased in only three patients, whereas prolidase activity was increased in 13. This could be due to a difference in the activation of these two enzymes in liver during the fibrotic process.

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This work was supported by grants from Unité d'Enseignement et de recherche Kremlin-Bicêtre, Université Paris-Sud (CR 809).

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