To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET.
Design:
Controlled clinical study.
Setting:
IVF unit, Department of Obstetrics and Gynecology, Carmel Medical Center.
Patient(s):
Patients with poor embryo quality in their previous IVF-ET cycles.
Intervention(s):
Embryos from 40 patients, in which >50% of the embryos were classified as poor quality in their previous IVF attempts, were grown on autologue GC culture system for a short period (24–48 hours) before being replaced in the uterine cavity.
Main Outcome Measure(s):
Embryo quality.
Result(s):
Significant decrease in poor quality embryos and increase in the proportion of good quality embryos were observed using a coculture system with autologue human GCs. Pregnancy rates in this groups of patients reached our standard IVF results during the same period.
Conclusion(s):
This study describes a simplified short-term coculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.
