Rapid communicationsFolate status, genomic DNA hypomethylation, and risk of colorectal adenoma and cancer: a case control study
Section snippets
Patients
Subjects were recruited from all patients referred for colonoscopy by the Department of Colorectal Surgery at King’s College Hospital between August 2000 and May 2001. Patients were recruited on the day of their colonoscopy, before the procedure. All patients underwent a clinically indicated colonoscopy for symptoms including rectal bleeding, change in bowel habit, and weight loss. Exclusion criteria included previous history of colorectal cancer, a strong family history of colorectal cancer
Results
Of the 201 patients approached, 196 patients agreed to participate in the study (98%). Of these, a total of 139 patients aged between 38 and 90 years were found to be suitable for inclusion: 28 patients with cancer, 35 patients with adenoma, and 76 controls. Their characteristics and clinical indications for colonoscopy are shown in Table 1. The most frequent indications for colonoscopy were change in bowel habit and rectal bleeding. Subjects with adenoma and cancer were older on average than
Discussion
The aim of this study was to investigate the hypothesis that genomic DNA methylation and folate status differ between subjects with colorectal adenoma and cancer and those with no colorectal abnormality. A folate status score was calculated for each subject based on 3 markers of folate status (folate intake, serum, and erythrocyte folate). We decided to use a combined score to avoid potential misclassification owing to limitations in both specificity and sensitivity associated with the methods
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