Alimentary TractIdentification of an autoimmune enteropathy–related 75-kilodalton antigen☆,☆☆,★
Section snippets
Sera
AIE sera were obtained from 2 Japanese and 2 German patients. The clinical features of the Japanese patients with X-linked AIE associated with tubulonephropathy (AIE 1 and 2) have been reported previously.5, 11, 12 The German patients were men with definite AIE without apparent renal complications (AIE 3 and 4). Control sera were obtained from normal volunteers (n = 10) or from patients with bronchial asthma (n = 20), food allergy (n = 3), autoimmune diseases including systemic lupus
Isolation of cDNA encoding an antigen reactive with AIE serum
Approximately 106 clones of a λgt11 human duodenal cDNA expression library were screened with the serum from 1 of the patients with AIE. Four clones reactive with the AIE serum but not with any of the control sera were obtained. From these clones, the most immunoreactive clone (D6) containing a 1023–base pair (bp) cDNA was isolated in the first screening. The cDNA insert was used as a probe to rescreen the same library, and 3 overlapping clones were obtained. Of these clones, K2 was the largest
Discussion
We have previously described a 75-kilodalton autoantigen that reacted with sera from 2 patients with AIE associated with tubulonephropathy.11 In the present study, we identified the AIE-specific 75-kilodalton autoantigen by cloning the cDNA from a human duodenal cDNA library and named it AIE-75. The AIE-75 cDNA consisted of 21 exons encoding 552 amino acids, and its calculated molecular weight was 62,172. However, by producing protein from AIE-75 cDNA in reticulocyte lysate, we found that
Acknowledgements
The authors thank Dr. N. Kurauchi (First Department of Surgery, Hokkaido University School of Medicine) for providing the tissue samples and Drs. A. Yara (Department of Pediatrics, University of the Ryukyu), M. Konno and A. Imamura (Sapporo Kohsei Hospital), N. Ishikawa (Kitami Red Cross Hospital), and B. Lembcke (St. Barbara Hospital, Gladbeck, Germany) for providing sera. The authors also thank M. Yanome for help in preparing the manuscript.
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Cited by (0)
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Address requests for reprints to: Ichiro Kobayashi, M.D., Ph.D., Department of Pediatrics, Hokkaido University School of Medicine, North-15 West-7, Kita-ku, Sapporo 060-8638 Japan. e-mail: [email protected]; fax: 81-11-706-7898.
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Supported in part by a Grant-in-Aid from the Ministry of Health and Welfare and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
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The nucleotide sequence reported in this study has been submitted to the DNA Data Bank of Japan with accession numbers AB006955 and AB018687.