In vitro bacterial penetration of coronally unsealed endodontically treated teeth

doi:10.1016/S0099-2399(07)80198-1

Journal of Endodontics

Volume 16, Issue 12, December 1990, Pages 566-569

https://doi.org/10.1016/S0099-2399(07)80198-1 Get rights and content

Forty-five root canals were cleaned, shaped, and then obturated with gutta-percha and root canal sealer, using a lateral condensation technique. The coronal portions of the root filling materials were placed in contact with Staphylococcus epidermidis and Proteus vulgaris. The number of days required for these bacteria to penetrate the entire root canals was determined. Over 50% of the root canals were completely contaminated after 19-day exposure to S. epidermidis. Fifty percent of the root canals were also totally contaminated when the coronal surfaces of their fillings were exposed to P. vulgaris for 42 days.

References (18)

SwansonKS et al.
An evaluation of coronal microleakage in endodontically treated teeth. Part I. Time periods
J Endodon
(1987)
MadisonS et al.
An evaluation of coronal microleakage in endodontically treated teeth. Part II. Sealer types
J Endodon
(1987)
MadisonS et al.
An evaluation of coronal microleakage in endodontically treated teeth. Part III. In vivo study
J Endodon
(1988)
EvansJT et al.
Evaluation of the apical seal produced by injected thermoplasticized gutta-percha in the absence of smear layer and root canal sealer
J Endodon
(1986)
HigginbothamTL
A comparative study of the physical properties of five commonly used root canal sealers
Oral Surg Oral Med Oral Pathol
(1967)
MaroskyJE et al.
Marginal leakage of temporary sealing materials used between endodontic appointments and assessed by calcium 45—an in vitro study
J Endodon
(1977)
AllisonDA et al.
The influence of the method of canal preparation on the quality of apical and coronal obturation
J Endodon
(1979)
MatloffIR et al.
A comparison of methods used in root canal sealability studies
Oral Surg
(1982)
KrakowAA et al.
In vivo study of temporary filling materials using in endontics in anterior teeth
Oral Surg
(1977)

There are more references available in the full text version of this article.

Cited by (440)

Methodologies used for testing the sealability of endodontic temporary fillings in vitro: A narrative review
2023, Saudi Dental Journal
The ability of the temporary filling to seal endodontic access cavities may be crucial for the success of endodontic treatment. Numerous in vitro studies have investigated the sealability of the temporary fillings used in endodontic treatments. However, in vitro sealability studies have been criticized for their inconsistent results and questionable clinical relevance. Some journals have imposed moratoriums on publishing such studies to encourage researchers to test their validity and clinical relevance. Since the implementation of this moratorium, little progress has been made in this field. To further encourage researchers to investigate the reliability of these studies, this review presents an overview of the methodologies of studies that examine the ability of temporary filling materials to seal the endodontic access cavity in vitro and discusses the criticisms of these studies in detail.
PubMed, Scopus, and Embase electronic databases were searched to identify studies that tested in vitro the ability of temporary filling materials to seal endodontic access cavities. Only original articles published in English between 01/01/1970 and 28/02/2022 were included.
The search yielded 551 results. After removing duplicates and excluding studies that did not meet the inclusion criteria, 94 studies were included in this review.
Although clinical studies may be the best way to test the performance of temporary fillings, the ethical importance of conducting preliminary in vitro studies is undeniable. It seems that questioning the reliability of in vitro sealability studies is not based on sufficient scientific evidence and that the inconsistencies in the results of these studies may be due to differences in the methodological and clinically relevant variables between them, rather than due to their unreliability.
Comparison of the sealing ability amongst orthograde apical plugs of mineral trioxide aggregate plus, mineral trioxide aggregate repair HP, and Biodentine after root resection: a bacterial leakage study
2024, Odontology
Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy
2024, Journal of Functional Biomaterials
Influence of Cementation Mode and Ferrule Design on the Fatigue Resistance of Monolithic Zirconia Endocrowns
2024, Journal of Clinical Medicine
Assessment of Periapical Status in Posterior Root Canal-Treated Teeth Using Cone Beam Computed Tomography in the Iranian Population
2023, Avicenna Journal of Dental Research
Evaluation of bacterial leakage and marginal adaptation of the bioceramics pulp dressing materials: an invitro study
2023, BMC Oral Health

View all citing articles on Scopus

¹: Dr. Torabinejad is director, Postgraduate Endodontics, Dr. Ung is a former graduate student in endodontics, and Dr. Kettering is professor of microbiology, Loma Linda University School of Medicine, Loma Linda, CA.

View full text

In vitro bacterial penetration of coronally unsealed endodontically treated teeth

J Endodon

J Endodon

J Endodon

J Endodon

Oral Surg Oral Med Oral Pathol

J Endodon

J Endodon

Oral Surg

Oral Surg