Methods and DevicesSEPARATION OF MALARIA-INFECTED ERYTHROCYTES FROM WHOLE BLOOD: USE OF A SELECTIVE HIGH-GRADIENT MAGNETIC SEPARATION TECHNIQUE
References (5)
- et al.
Direct magnetic separation of red cells from whole blood
Nature
(1975) - et al.
Differential blood cell separation using a high gradient magnetic field
Br J Haematol
(1978)
Cited by (124)
Magnetic cell separation
2021, Magnetic Materials and Technologies for Medical ApplicationsAntibody-free rapid diagnosis of malaria in whole blood with surface-enhanced Raman Spectroscopy using Nanostructured Gold Substrate
2020, Advances in Medical SciencesCitation Excerpt :It is therefore critical to improve the sensitivity to detect free hemozoin in plasma and parasite-containing cells. Recent studies mainly used cultured P. falciparum infected cells as samples, as in vitro cultured P. falciparum has a higher pathogen concentration and a high ratio of trophozoite and schizont stage, instead of real blood samples from patients [7,10,11]. Besides, P. vivax - commonly found in southeast Asia - suggests that Raman detection of different malarial types is needed prior to clinical application [18].
Magnetic Susceptibility Difference-Induced Nucleus Positioning in Gradient Ultrahigh Magnetic Field
2020, Biophysical JournalBiological activity and stability analyses of knipholone anthrone, a phenyl anthraquinone derivative isolated from Kniphofia foliosa Hochst.
2019, Journal of Pharmaceutical and Biomedical AnalysisCitation Excerpt :Synchronization of P. falciparum parasites was carried out with 5% (w/v) sorbitol [9]. P. falciparum trophozoites were enriched by magnet separation [10]. The half maximal effective concentration (EC50) of antimalarial drugs on P. falciparum 3D7 asexual blood stages was determined by the [3H] hypoxanthine incorporation assay as described elsewhere [11].
The paramagnetic properties of malaria pigment, hemozoin, yield clues to a low-cost system for its trapping and determination
2019, TalantaCitation Excerpt :Hemozoin, a large paramagnetic crystal pigment, is generated by Plasmodium through a detoxification pathway, the polymerization of iron III protoporphyrin-IX ring complexes released through hemoglobin digestion [10,11]; a full blood count technology incorporating analysis of depolarized laser light has been proposed to detect hemozoin in monocytes and neutrophils [12], a possible automated malaria detection system but that has not been further developed. Recently, the levels of hemozoin-containing red blood cells (RBCs) [13–16] and of total blood hemozoin [17] have been proposed as a measurement of parasitemia to diagnose malaria. To this end, different microscopic and instrumental methods have been developed; in particular, a magneto-optical method achieves detectability of 50–100 parasites/µL [16] but the necessary equipment (large electro-magnet, laser, sophisticated optics…) probably limits its use in developing countries where demand is the highest.