Trends in Genetics
Genome analysisHuman housekeeping genes are compact
Section snippets
Assignment of housekeeping genes
A recently published database provides microarray expression data for Affymetrix U95A chip, containing 12 600 probes, and hybridized to 101 different samples [9] from 47 different human tissues and cell lines. These samples are mainly from the normal human physiological state, and therefore this dataset provides a description of the normal mammalian transcriptome.
We calculated the distribution of the number of different tissues in which a gene is expressed. Discarding probes for which the
Length analysis of HK genes
Table 1 compares the lengths of various parts of the HK genes and the background genes. The alignment data was taken from the UCSC genome browser (http://genome.ucsc.edu) [16]. We excluded 322 genes that do not have a unique alignment, as well as 1242 genes that were not expressed in any tissue (to avoid potential problems because of defective probes). This left 532 HK genes and 5404 non-HK genes. The histograms in Fig. 1b–d compare HK genes with the other genes by total intron length, 5′ UTR
Acknowledgements
We thank Andrew Su for helpful discussion and for providing us with the RefSeq mapping. Gady Cojocaru and Rotem Sorek are acknowledged for comments on the manuscript and insightful discussion.
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