Cell kinetics in mandibular ameloblastic fibro-odontoma evaluated by bromodeoxyuridine and proliferating cell nuclear antigen immunohistochemistry: case report

Abstract

A case of ameloblastic fibro-odontoma in a 10-year-old Japanese male patient is presented. Bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) immunohistochemistry were applied to investigate the cell kinetics of this lesion. The tumour proliferation activity was individually evaluated through the labelling index (LI) at the epithelial and mesenchymal components. The LI of BrdU was 0.9% in the epithelial and 2.1% in the mesenchymal component, while the LI of PCNA was 2.2% in the epithelial and 7.7% in the mesenchymal component. These immunohistochemical investigations suggest that the mesenchymal component was more proliferative than the epithelial component. Such an immunohistochemical investigation may be useful in evaluating the proliferative potential of odontogenic tumours.