Elsevier

Neuroscience Letters

Volume 319, Issue 1, 8 February 2002, Pages 53-57
Neuroscience Letters

Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus

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Abstract

The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers.

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Acknowledgements

The authors wish to thank the Department of Medical Microbiology, University of Aberdeen for assistance in sequencing analysis, Dr David Julius for the gift of VR1 antibody, Novartis for VR1 fusion protein and The Wellcome Trust (Grant No. 55437) for financial support.

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