The Calcium Binding Protein Calretinin is a Selective Marker for Malignant Pleural Mesotheliomas of the Epithelial Type

doi:10.1016/S0344-0338(96)80208-1

Pathology - Research and Practice

Volume 192, Issue 2, 1996, Pages 137-147

https://doi.org/10.1016/S0344-0338(96)80208-1 Get rights and content

Summary

In a series of 23 cases of mesothelioma of either the epithelial, sarcomatoid or the mixed type, the expression of three calcium-binding proteins (calretinin, parvalbumin and calbindin-D28k) was studied using immunohistochemical techniques on paraffin sections. The results show that calretinin is expressed in mesotheliomas of the epithelial type (papillary, adenomatous or solid) and by the epithelial component of the mixed tumours. The immunohistochemical reaction is specific and reproducible. The tissues of the pulmonary parenchyma and of the pleura are negative for calretinin except for the rare fibroblasts and some skeletal muscle fibres situated in the interstices of, or near the epithelial tumour mass. The sarcomatoid mesotheliomas and the sarcomatoid component of the mixed tumours do not express calretinin. Parvalbumin and calbindin-D28k are expressed neither in mesotheliomas nor in normal lung tissue. Primary adenocarcinomas of the lung are negative for all three calcium binding proteins cited. Thus, calretinin seems to represent a selective marker for mesotheliomas of the epithelial type and allows their differentiation from metastases of lung adenocarcinomas.

References (52)

KG Baimbridge et al.
Calciumbinding proteins in the nervous system
TINS
(1992)
MR Celio et al.
Monoclonal antibodies against calbindin D-28k
Cell Calcium
(1990)
JG Chafouleas et al.
Calmodulin and the cell cycle : involvement in regulation of cell-cycle progression
Cell
(1982)
JP Durkin et al.
The src gene product (pp^60src) of avian sarcoma virus rapidly induces DNA synthesis and proliferation of calcium-depleted rat cells
Biochem Biophys Res Commun
(1981)
V Gotzos et al.
Expression of the calcium binding protein calretinin in WiDr cells and its correlation to their cell cycle
Expt Cell Res
(1992)
PK Hepler
Calcium and Mitosis
Int Rev Cyt
(1992)
U Kägi et al.
Ca²⁺-binding parvalbumin in rat testis. Characterization, localisation, and expression during development
J Biol Chern
(1987)
E Kiràly et al.
In vitro detection of calretinin immunoreactivity in chicken embryo dorsal root ganglion neurons: a possible developmental marker
Dev Brain Res
(1993)
M Klug et al.
Intracellular Ca²⁺ and Ca²⁺-binding proteins in chemically transformed rat fibroblasts
Exp Cell Res
(1994)
B Schwaller et al.
Characterization of a polyclonal antiserum against the purified human recombinant calcium binding protein calretinin
Cell Calcium
(1993)

K Sheibani

Immunopathology of malignant mesothelioma

Hum Pathol

(1994)

KI Strauss et al.

Calretinin is expressed in the Leydig cells of rat testis

Biochim Biophys Acta

(1994)

ML Veigl et al.

Calcium and calmodulin in cell growth and transformation

Biochim Biophys Acta

(1984)

DM Vogt Weisenhorn et al.

Localisation of calretinin in cells of layer I (Cajal-Retzius cells) of the developing cortex of the rat

Dev Brain Res

(1994)

MR Wick et al.

Malignant epithelioid pleural mesothelioma versus peripheral pulmonary adenocarcinoma: a histochemical, ultrastructural, and immunohistologic study of 103 cases

Hum Pathol

(1990)

L Zeng et al.

Immunocytochemical characterization of cell lines from human malignant mesothelioma: characterization of human mesothelioma cell lines by immunocytochemistry with a panel of monoclonal antibodies

Hum Pathol

(1994)

Ch Andressen et al.

Calcium-binding proteins: selective markers of nerve cells

Cell Tissue Res

(1993)

YS Babu et al.

Three-dimensional structure of calmodulin

Nature

(1985)

CWM Bedrossian et al.

Differential diagnosis between mesothelioma and adenocarcinoma: a multimodal approach based on ultrastructure and immunocytochemistry

Seminars in Diagnostic Pathology

(1992)

PhT Cagle et al.

Immunohistochemical differentiation of sarcomatoid mesotheliomas from other spindle cell neoplasms

Am J Clin Pathol

(1989)

E Carafoli

Intracellular calcium homeostasis

Ann Rev Biochem

(1987)

TCK Chan

Calcium-independent growth of human ovarian carcinoma cells

J Cell Physiol

(1989)

K Chang et al.

Monoclonal antibody KI reacts with epithelial mesothelioma but not with lung adenocarcinoma

Am J Surg Pathol

(1992)

P Cohen

Protein phosphorylation and hormone action

Proc R Soc Lond (Biol)

(1988)

RS Cotran et al.

JE Craighead et al.

The pathogenesis of malignant and nonmalignant serosal lesions in body cavities consequent to asbestos exposure.

Cited by (128)

Malignant and Borderline Mesothelial Tumors of the Pleura
2018, Practical Pulmonary Pathology: A Diagnostic Approach A Volume in the Pattern Recognition Series
This chapter considers the clinicopathologic features of borderline and malignant mesothelial pleural neoplasms. They include “well-differentiated papillary mesothelioma,” multicystic mesothelial tumors of borderline biological potential, and malignant mesotheliomas. The specialized morphological features of those lesions are discussed, with emphasis on differential diagnosis with other tumors.
Toll/IL-1 domain-containing adaptor inducing IFN-β (TRIF) mediates innate immune responses in murine peritoneal mesothelial cells through TLR3 and TLR4 stimulation
2016, Cytokine
Citation Excerpt :
Values of P < 0.05 were considered significant. To determine phenotypic characteristics of PMCs, the expression of calretinin that is known as a selective marker for mesothelial cells [27,28] was evaluated by Western blot. Calretinin was strongly expressed in PMCs, but there was no expression in BMDMs (Fig. 1a).
Mesothelial cells are composed of monolayer of the entire surface of serosal cavities including pleural, pericardial, and peritoneal cavity. Although mesothelial cells are known to express multiple Toll-like receptors (TLRs) which contribute to trigger innate immune responses against infections, the precise molecular mechanism remains still unclear. In the present study, we investigated the role of Toll/IL-1 domain-containing adaptor inducing IFN-β (TRIF), one of the two major TLRs–adaptor molecules, on innate immune response induced by TLR3 and TLR4 stimulation in murine peritoneal mesothelial cells (PMCs). TRIF was strongly expressed in PMCs and its deficiency led to impaired production of cytokines and chemokines by poly I:C and LPS in the cells. Activation of NF-κB or MAPKs through poly I:C and LPS stimulation was reduced in TRIF-deficient PMCs as compared to the WT cells. TRIF was also necessary for optimal nitric oxide synthesis and gene expression of inducible nitric oxide synthase (iNOS) and IFN-β in PMCs in response to poly I:C and LPS. Furthermore, both Escherichia coli and Pseudomonas aeruginosa induced high level of IL-6, CXCL1, and CCL2 production in PMCs, which was significantly impaired by TRIF deficiency. These results demonstrated that TRIF is required for optimal activation of innate immune responses in mesothelial cells against microbial infections.
The established and future biomarkers of malignant pleural mesothelioma
2015, Cancer Treatment Reviews
Malignant pleural mesothelioma (MPM) is an asbestos-related cancer with a median survival of 12 months. The MPM incidence is 1–6/100,000 and is increasing as a result of historic asbestos exposure in industrialized countries and continued use of asbestos in developing countries. Lack of accurate biomarkers makes diagnosis, prognostication and treatment prediction of MPM challenging. The aim of this review is to identify the front line of MPM biomarkers with current or potential clinical impact. Literature search using the PubMed and PLoS One databases, the related-articles function of PubMed and the reference lists of associated publications until April 26th 2015 revealed a plethora of candidate biomarkers. The current gold standard of MPM diagnosis is a combination of two positive and two negative immunohistochemical markers in the epithelioid and biphasic type, but sarcomatous type do not have specific markers, making diagnosis more difficult. Mesothelin in serum and pleural fluid may serve as adjuvant diagnostic with high specificity but low sensitivity. Circulating proteomic and microRNA signatures, fibulin-3, tumor cell gene-ratio test, transcriptomic, lncRNA, glycopeptides, pleural fluid FISH assay, hyaluronate/N-ERC mesothelin and deformability cytometry may be important future markers. Putative predictive markers for pemetrexed–platinum are tumor TS and TYMS, for vinorelbine the ERCC1, beta-tubuline class III and BRCA1. Mutations of the BAP1 gene are potential markers of MPM susceptibility. In conclusion, the current status of MPM biomarkers is not satisfactory but encouraging as more sensitive and specific non-invasive markers are emerging. However, prospective validation is needed before clinical application.
Pericardial malignant mesothelioma diagnosed in a dog by immunocytochemistry of the pericardial fluid: a case report
2023, BMC Veterinary Research
Research progress on immunohistochemical diagnostic markers for malignant pleural mesothelioma
2023, Chinese Journal of Industrial Hygiene and Occupational Diseases
DNA repair and damage pathways in mesothelioma development and therapy
2022, Cancer Cell International

View all citing articles on Scopus

^*: Prof. Dr. V. Gotzos, Institute of Histology and General Embryology, University of Fribourg, CH-1705 Fribourg, Switzerland

View full text

The Calcium Binding Protein Calretinin is a Selective Marker for Malignant Pleural Mesotheliomas of the Epithelial Type

Summary

TINS

Cell Calcium

Cell

Biochem Biophys Res Commun

Expt Cell Res

Int Rev Cyt

J Biol Chern

Dev Brain Res

Exp Cell Res

Cell Calcium

Hum Pathol

Biochim Biophys Acta

Biochim Biophys Acta

Dev Brain Res

Hum Pathol

Hum Pathol

Calcium-binding proteins: selective markers of nerve cells

Cell Tissue Res

Three-dimensional structure of calmodulin

Nature

Differential diagnosis between mesothelioma and adenocarcinoma: a multimodal approach based on ultrastructure and immunocytochemistry

Seminars in Diagnostic Pathology

Immunohistochemical differentiation of sarcomatoid mesotheliomas from other spindle cell neoplasms

Am J Clin Pathol

Intracellular calcium homeostasis

Ann Rev Biochem

Calcium-independent growth of human ovarian carcinoma cells

J Cell Physiol

Monoclonal antibody KI reacts with epithelial mesothelioma but not with lung adenocarcinoma

Am J Surg Pathol

Protein phosphorylation and hormone action

Proc R Soc Lond (Biol)

The pathogenesis of malignant and nonmalignant serosal lesions in body cavities consequent to asbestos exposure.