Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
EndodonticsEfficacy of chlorhexidine- and calcium hydroxide–containing medicaments against Enterococcus faecalis in vitro
Section snippets
Agar diffusion test
E faecalis (ATCC 292121) and Streptococcus mutans (NG8) were grown overnight in brain-heart infusion (BHI) broth (Difco Laboratories, Baltimore, Md), adjusted to a 0.5 turbidity reading on the McFarland scale (1.5 × 108 bacteria/mL), and inoculated in BHI agar plates. Inoculation was performed by using sterile pipettes brushed across the media.27 Round wells, 5 mm deep and 6 mm in diameter, were punched in the cultivated agar plates and filled with one of the test medicaments or controls. The
Agar diffusion test
Excluding the control groups, there was a very significant correlation between the zones of inhibition against E faecalis and S mutans (r = .930; P < .001). S mutans was inhibited by all the medicaments, and E faecalis was inhibited by all the CHX-containing medicaments (Fig 2). For all the medicaments, S mutans was inhibited significantly better than E faecalis was (P < .05). Neither control group demonstrated any inhibitory effect against either bacteria (data not shown).
In the
Discussion
The experimental model used in this study was adapted from that established by Ørstavik and Haapasalo15 for infection and disinfection of dentinal tubules. As in our previous study,26 the model was adapted for extracted human teeth rather than bovine incisors. This modification was considered appropriate because of the marked difference in diameter between the canals of bovine and human teeth and thus in the volume of medicament that can be placed in these canals.
E faecalis was chosen for the
Acknowledgements
We thank Dr Dennis Cvitkovitch and Mrs Kristen Krastel for their valuable support in conducting the microbiologic procedures of this study.
Assistant Professor and Head, Endodontic Division, Department of Dental Clinical Sciences, Dalhousie University, Halifax, Nova Scotia, Canada
References (47)
- et al.
Prevalence of black-pigmented bacteroides species in root canal infections
J Endod
(1989) - et al.
Factors affecting the long-term results of endodontic treatment
J Endod
(1990) - et al.
Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesionsa long-term light and electron microscopic follow-up study
J Endod
(1990) - et al.
Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy
Oral Surg Oral Med Oral Pathol
(1983) - et al.
The effect of chlorhexidine gluconate irrigation on the root canal flora of freshly extracted necrotic teeth
Oral Surg Oral Med Oral Pathol
(1982) - et al.
A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants
J Endod
(1994) - et al.
Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria
J Endod
(1998) - et al.
Residual antimicrobial activity after canal irrigation with chlorhexidine
J Endod
(1997) - et al.
Antimicrobial substantivity of chlorhexidine-treated bovine root dentin
J Endod
(2000) - et al.
Antimicrobial substantivity of bovine root dentin exposed to different chlorhexidine delivery vehicles
J Endod
(2000)
Substantive antimicrobial activity in chlorhexidine-treated human root dentin
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Evaluation of the antibacterial activities of calcium hydroxide, chlorhexidine, and camphorated paramonochlorophenol as intracanal medicament. A clinical and laboratory study
J Endod
Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals
J Endod
The effectiveness of various disinfectants used as endodontic intracanal medicationsan in vitro study
J Endod
Antimicrobial and toxic effects of established and potential root canal irrigants
J Endod
A comparison of the antimicrobial efficacy of three calcium hydroxide formulations on human dentin infected with Enterococcus faecalis
J Endod
Uptake and release of chlorhexidine by bovine pulp and dentin specimens and their subsequent acquisition of antibacterial properties
Oral Surg Oral Med Oral Pathol
The effects of surgical exposures of dental pulps in germfree and conventional laboratory rats
J South Calif Dent Assoc
Micro-organisms from necrotic pulp of traumatized teeth
Odontol Revy
Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy
Scand J Dent Res
Studies into the microbial spectrum of apical periodontitis
Int Endod J
Evaluation of root canal bacteria and their susceptibility in teeth with necrotic pulp
Oral Microbiol Immunol
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Assistant Professor and Head, Endodontic Division, Department of Dental Clinical Sciences, Dalhousie University, Halifax, Nova Scotia, Canada
Assistant Professor, Institute of Dentistry, University of Helsinki; Department of Oral and Maxillofacial Diseases, University Central Hospital; and Helsinki City Health Department, Helsinki, Finland
Assistant Professor, Department of Dentistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
Assistant Professor, Department of Endodontics, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
Senior Lecturer, Department of Pharmacology, Faculty of Medicine and Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
Assistant Professor, Department of Community Dentistry, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada
Professor and Head, Department of Endodontics. Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada