Article
High-magnification ICSI overcomes paternal effect resistant to conventional ICSI

https://doi.org/10.1016/S1472-6483(10)60975-3Get rights and content

Abstract

Previous studies have shown that repeated intracytoplasmic sperm injection (ICSI) failures can be caused by a paternal effect. Other studies have suggested that ICSI results are compromised if morphologically abnormal spermatozoa are injected into oocytes. This study was undertaken to evaluate the usefulness of a high-magnification optical system to select spermatozoa to be used for ICSI (high-magnification ICSI) in couples with repeated conventional ICSI failures. Couples with two or more previous conventional ICSI failures underwent an additional conventional ICSI attempt, followed by a high-magnification ICSI attempt. The outcomes of the two sequential attempts were compared. In 72 of these patients, sperm DNA integrity was assessed. In the whole group of 125 couples with repeated ICSI failures, high-magnification ICSI improved clinical outcomes (pregnancy, implantation, delivery and birth rates) without affecting biological outcomes (fertilization and cleavage rates, embryo morphology). The improvement of clinical ICSI outcomes was evident both in patients with an elevated degree of sperm DNA fragmentation and in those with normal sperm DNA status. It is concluded that high-magnification ICSI improves clinical outcomes in couples with previous repeated conventional ICSI failures.

Section snippets

André Hazout was co-leader of the ART programme of Frydman's team in Clamart 1983–2003. Currently he leads the ART programme of Bichat Hospital in the University Paris VII and the Private Reproductive Medicine and Infertility Unit of ‘Eylau La Muette’ in Paris. He is Vice President of the French Society of Reproductive Medicine. He was responsible for development of the Hazout oocyte collector and has published extensively in both national and international journals. His recent research

References (32)

  • D Sakkas et al.

    Abnormal spermatozoa in the ejaculate: abortive apoptosis and faulty nuclear remodelling during spermatogenesis

    Reproductive BioMedicine Online

    (2003)
  • N Sukcharoen et al.

    Sperm morphology evaluated by computer (IVOS) cannot predict the fertilization rate in vitro after intracytoplasmic sperm injection

    Fertility and Sterility

    (1998)
  • J Tesarik

    Paternal effects on cell division in the human preimplantation embryo

    Reproductive BioMedicine Online

    (2005)
  • B Bartoov et al.

    Real-time fine morphology of motile human sperm cells is associated with IVF–ICSI outcome

    Journal of Andrology

    (2002)
  • A Berkovitz et al.

    The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm

    Human Reproduction

    (2005)
  • E Greco et al.

    Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment

    Journal of Andrology

    (2005)
  • Cited by (0)

    André Hazout was co-leader of the ART programme of Frydman's team in Clamart 1983–2003. Currently he leads the ART programme of Bichat Hospital in the University Paris VII and the Private Reproductive Medicine and Infertility Unit of ‘Eylau La Muette’ in Paris. He is Vice President of the French Society of Reproductive Medicine. He was responsible for development of the Hazout oocyte collector and has published extensively in both national and international journals. His recent research interests include the role of somatostatin in the treatment of male infertility, the proteomic profile of the seminal plasma of infertile men, sclerotherapy of endometriosis cysts before ART, and embryo implantation markers in endothelium explants.

    View full text