Article
Effect of gonadotrophin stimulation on mouse oocyte quality and subsequent embryonic development in vitro

https://doi.org/10.1016/S1472-6483(10)61002-4Get rights and content

Abstract

In-vivo-matured oocytes were collected from naturally ovulated and superovulated [pregnant mare's serum gonadotrophin (PMSG) + human chorionic gonadotrophin (HCG)] mice. Immature oocytes were retrieved from naturally cycling mice and from mice primed with PMSG. The percentages of cleavage and blastocyst formation were significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes. Blastocyst formation rate was significantly higher (P < 0.05) in immature oocytes derived from PMSG-primed mice, and the percentages of oocytes with comet tails, and their length, were significantly higher and longer respectively in in-vitro-matured oocytes. Total cell numbers of blastocysts were also significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes, but there were also no differences in ratio of trophectoderm (TE)/inner cell mass (ICM). In conclusion, in-vivo-matured mouse oocytes were more competent than those matured in-vitro, perhaps due to a lesser degree of DNA damage. Embryonic development capacity of in-vivo-matured oocytes is not promoted by ovarian stimulation. Gonadotrophin priming prior to immature mouse oocyte retrieval is beneficial to subsequent embryonic development.

Section snippets

Yue Wang obtained her Bachelor of Medicine (1997) and Master of Science (2002) degrees in Norman Bethune Medical University (merged to Jilin University as School of Medicine), China. Currently she is pursuing post-graduate study towards the PhD degree under Dr Ri-Cheng Chian's supervision at the Division of Experimental Medicine, McGill University, located at Montreal, Canada. Her thesis project is comparing the differences between oocytes matured in vivo and in vitro, using the mouse model.

References (69)

  • EG Papanikolaou et al.

    Immature oocyte in-vitro maturation: clinical aspects

    Reproductive BioMedicine Online

    (2005)
  • GS Prins et al.

    Gonadotropins augment maturation and fertilization of human immature oocytes cultured in vitro

    Fertility and Sterility

    (1987)
  • P Quinn et al.

    Successful human in-vitro fertilization using a modified human tubal fluid medium lacking glucose and phosphate ions

    Fertility and Sterility

    (1995)
  • C Racowsky et al.

    Embryo quality based on ovulation induction: defining the differences

    Reproductive BioMedicine Online

    (2005)
  • D Rizos et al.

    Effect of culture system on the yield and quality of bovine blastocysts as assessed by survival after vitrification

    Theriogenenology

    (2001)
  • AC Schroeder et al.

    The developmental capacity of mouse oocytes that matured spontaneously in-vitro is normal

    Developmental Biology

    (1984)
  • NP Singh et al.

    A simple technique for quantitation of low levels of DNA damage in individual cells

    Experimental Cell Research

    (1988)
  • GA Thouas et al.

    Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts

    Reproductive BioMedicine Online

    (2001)
  • LL Veeck et al.

    Maturation and fertilization of morphologically immature human oocytes in a program of in-vitro fertilization

    Fertility and Sterility

    (1983)
  • S Von Otte et al.

    Lessons learned from introducing an in-vitro maturation programme into clinical practice

    Reproductive BioMedicine Online

    (2005)
  • R Boni et al.

    Intercellular communication in in vivo- and in vitro-produced bovine embryos

    Biology of Reproduction

    (1999)
  • RC Chian et al.

    State of the art in in-vitro maturation

    Current Opinion in Obstetrics and Gynecology

    (2004)
  • CM Combelles et al.

    Assessment of oocyte quality following repeated gonadotropin stimulation in the mouse

    Biology of Reproduction

    (2003)
  • P De Sutter et al.

    Chromosome analysis in human oocytes unfertilized in vitro: a mathematical model for the estimation of the first meiotic non-disjunction frequency

    Human Reproduction

    (1991)
  • J Delhanty et al.

    The origin of genetic defects in the human and their detection in the preimplantation embryo

    Human Reproduction Update

    (1995)
  • WR Edirisinghe et al.

    Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection

    Human Reproduction

    (1997)
  • S El Mouatassim et al.

    Expression of gene encoding antioxidant enzymes in human and mouse oocytes during the final stages of maturation

    Molecular Human Reproduction

    (1999)
  • JJ Eppig et al.

    Development in vitro of mouse oocyte from primordial follicles

    Biology of Reproduction

    (1996)
  • JJ Eppig et al.

    Developmental capacity of mouse oocytes matured in vitro: effects of gonadotropic stimulation, follicular origin, and oocyte size

    Journal of Reproduction and Fertility

    (1992)
  • G Ertzeid et al.

    The impact of ovarian stimulation on implantation and fetal development in mice

    Human Reproduction

    (2001)
  • G Ertzeid et al.

    Adverse effects of gonadotrophin treatment on pre- and postimplantation development in mice

    Journal of Reproduction and Fertility

    (1992)
  • T Fair et al.

    Ultrastructure of bovine blastocysts following cryopreservation: effect of method of embryo production on blastocyst quality

    Molecular Reproduction and Development

    (2001)
  • A Gougeon

    Regulation of ovarian follicular development in primates: facts and hypotheses

    Endocrine Reviews

    (1996)
  • L Gras et al.

    The incidence of chromosomal aneuploidy in stimulated and unstimulated (natural) uninseminated human oocytes

    Human Reproduction

    (1992)
  • Cited by (0)

    Yue Wang obtained her Bachelor of Medicine (1997) and Master of Science (2002) degrees in Norman Bethune Medical University (merged to Jilin University as School of Medicine), China. Currently she is pursuing post-graduate study towards the PhD degree under Dr Ri-Cheng Chian's supervision at the Division of Experimental Medicine, McGill University, located at Montreal, Canada. Her thesis project is comparing the differences between oocytes matured in vivo and in vitro, using the mouse model.

    View full text