Development of an LC–MS–MS method for the quantification of taurine derivatives in marine invertebrates
Section snippets
Reagents
Taurine (NH2–CH2–CH2–SO3H) and hypotaurine (NH2–CH2–CH2–SO2H) were purchased from Sigma, 2-aminoethyl hydrogenosulfuric acid (NH2–CH2–CH2–O–SO3H) was purchased from Fluka, and 2-aminoethylthiosulfonic acid (NH2–CH2–CH2–S–SO3H) was purchased from Aldrich. Pure crystalline thiotaurine (NH2–CH2–CH2–SO2SH) was prepared according to the method of Cavallini et al. [32], whereby a solution of hypotaurine in 0.2 N NaOH is reacted with elemental sulfur in ethanol for 2 h at 85 °C. The solution is then
Results and discussion
Taurine-like compounds (Table 1) can be considered as amino acids. Thus, LC–MS–MS was investigated to replace the lengthy traditional LC–UV or fluorescence methods using pre- or postderivatization (mainly ortho-phthaldialdehyde) of the analytes. The first step in the development of the LC–MS or LC–MS–MS analytical methodology was the investigation of the most specific and sensitive conditions for detection (e.g., ionization mode, fragmentation).
Conclusion
The goal of this study was to develop a reliable analytical method to characterize and quantify unusual sulfur amino compounds, especially thiotaurine, found in symbiotic deep marine invertebrates. Coupling LC with tandem MS proves to be a reliable procedure for the identification and quantification of metabolites in biological samples given that MS–MS detection is a highly sensitive and specific detection mode, whereas LC provides selectivity between the remaining isobars or isomers contained
Acknowledgments
We thank the scientific teams and the crew of the joint French–U.S. hydrothermal vent cruise Hydronaut for their assistance in collecting material. This study was partially supported by a financial Grant (02/CNES/4800000093) from the Centre National d’Etudes Spatiales (CNES).
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