Biochimica et Biophysica Acta (BBA) - Biomembranes
Volume 1818, Issue 2, February 2012, Pages 225-233
Artificial membrane-like environments for in vitro studies of purified G-protein coupled receptors☆
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Graphical abstract
Highlights
► Reconstitution into native membrane-like environments is needed for quantitative biophysical studies of GPCRs. ► Six classes of membrane mimics are reviewed and evaluated for GPCR reconstitution. ► Classes reviewed here include micelles, bicelles, nanodiscs, lipid vesicles, planar lipid membranes, and lipidic cubic phases. ► Theoretical advantages and limitations for each reconstitution system are discussed. ► Examples of applications are provided.
Abbreviations
GPCR
G-protein coupled receptor
SUV
small unilamellar vesicle
GUV
giant unilamellar vesicle
MSP
membrane scaffold protein
PLM
planar lipid membrane
Keywords
GPCR reconstitution
Bicelle
Nanodisc
Giant unilamellar vesicle
Planar lipid membrane
Lipidic cubic phase
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This article is part of a Special Issue entitled: Membrane protein structure and function.
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