Biochemical and Biophysical Research Communications
A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver☆,☆☆
Section snippets
Materials and methods
Mice. C57BL6/Tg14 (act-EGFP) OsbY01 mice (GFP-Tg mice) showed GFP expression in multiple tissue and cells and were kindly provided by Masaru Okabe (Genome Research Center, Osaka University, Osaka, Japan) [13]. C57BL/6 female mice were purchased from Japan SLC (Shizuoka, Japan). AML1 knockout mice were generated as described previously [24]. The genetic background of these mice used in this study was C57BL/6 mice. Male and female mice were mated overnight and female mice were scored based on
Anti-Liv8 antibody detected hematopoietic progenitor cell in fetal liver at E 11.5
Previously we had raised a rat monoclonal antibody, anti-Liv2, which recognized hepatoblasts at E 9.5 [21]. As shown in Fig. 1A, Liv2-positive cells were also detected in fetal liver at E 11.5. Using the antibody developed in this study, Liv8-positive cells were seen in the fetal liver on E 11.5 (Fig. 1B). Fetal liver at E 11.5 functions as a secondary hematopoietic organ [17]. We analyzed whether anti-Liv8 positive cell is associated with hepatoblast or hematopoietic cell. We found
Discussion
The anti-Liv8 antibody is a useful antibody to separate hematopoietic cells and non-hematopoietic cells in adult bone marrow. We found Liv8-positive cells in fetal liver at E11.5, but could not detect no-positive cells in fetal liver of AML1 knockout mice (Fig. 1C) at E 11.5. This result suggested that anti-Liv8-positive cell might be associated with the generation of HSC. We used FACS analysis to understand more about the characterization of Liv8-positive cells in the bone marrow. Around 32%
Acknowledgements
We thank Dr. Masaru Okabe (Genome Research Center, Osaka University) for the gift of GFP transgenic mice and Mr. Jun Oba for his excellent support for immunohistochemistry.
References (34)
- et al.
Liver from bone marrow in humans
Hepatology
(2000) - et al.
Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell
Cell
(2001) - et al.
Plasticity of marrow-derived stem cells
Blood
(2003) - et al.
‘Green mice’ as a source of ubiquitous green cells
FEBS Lett.
(1997) - et al.
Albumin-expressing hepatocyte-like cells develop in the livers of immune-deficient mice that received transplants of highly purified human hematopoietic stem cells
Blood
(2003) - et al.
Cytokine regulation of liver development
Biochim. Biophys. Acta
(2002) - et al.
Functional heterogeneity of the hematopoietic microenvironment: rare stromal elements maintain long-term repopulating stem cells
Blood
(1996) - et al.
SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis
Dev. Biol.
(2002) - et al.
AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis
Cell
(1996) - et al.
Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo
Blood
(2003)
Anti-CD45-mediated cytoreduction to facilitate allogeneic stem cell transplantation
Blood
Establishment of Alb-DsRed2 transgenic rat for liver regeneration research
Biochem. Biophys. Res. Commun.
Bone marrow as a potential source of hepatic oval cells
Science
Hepatocytes from non-hepatic adult stem cells
Nature
Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract
Nat. Med.
Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells
N. Engl. J. Med.
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Abbreviations: BMC, bone marrow cell; CCl4, carbon tetrachloride; FAH, fumarylacetoacetate hydrolase; GFP, green fluorescent protein; EGFP, enhanced GFP; GFP-Tg mice, C57BL6/Tg14 (act-EGFP) OsbY01 mice; HSC, hematopoietic stem cell; E, embryonic day; MSC, mesenchymal stem cells; MAPC, multipotent adult progenitor cell.
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This work was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 13470121 to Shuji Terai, Isao Sakaida, and Kiwamu Okita, and No. 13770262 to Shuji Terai) for translational research from the Ministry of Health, Labor and Welfare (H-trans-5 to Shuji Terai, Isao Sakaida, Hiroshi Nishina, and Kiwamu Okita).
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Request for Anti-Liv8 contact to Dr. Hiroshi Nishina, Department of Physiological Chemistry, Graduate School of Pharmaceutical Science, University of Tokyo, Hongo 7-3-1, Bunkyoku, Tokyo 113 0033, Japan.