Renal carcinoma cells undergo apoptosis without oligonucleosomal DNA fragmentation
Section snippets
Materials and methods
Cell lines and culture conditions. RCC cell line RCC-91 was established from a surgical specimen at the Cleveland Clinic Foundation (Cleveland, OH). Jurkat cells were obtained from the American Type Culture Collection (Manassas, VA). Cells were cultured in RPMI 1640 (Bio-Whittaker, Walkersville, MD) supplemented with 10% FCS (Hyclone, Logan, UT), l-glutamine (2 mM), gentamicin (50 mg/L), sodium pyruvate (1 mM), and non-essential amino acids (0.1 mM).
Antibodies and reagents. Antibodies to caspase-3
Results and discussion
Apoptosis is a specific process leading to programmed cell death through the activation of evolutionarily conserved intracellular pathways. Apoptotic cell death is characterized by a series of unique biochemical and morphological events such as proteolytic activation of the caspase family members, cleavage of multiple proteins, phosphatidylserine exposure, cell shrinkage, condensation and fragmentation of chromatin, and oligonucleosomal DNA fragmentation [1], [2], [3], [4], [7]. Renal cell
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Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease- mediated 3′-OH single-strand DNA breaks
2013, Journal of Biological ChemistryCitation Excerpt :Indeed, genetically modified CAD−/− DT40 chicken cells do not reach stage II chromatin condensation after apoptotic stimuli (17). Conversely, some studies indicate that stage II chromatin condensation and the oligonucleosomal DNA degradation processes can occur separately (18–23). Therefore, how DFF40/CAD endonuclease influences stage II chromatin condensation during caspase-dependent apoptotic cell death still remains elusive.
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