Biochemical and Biophysical Research Communications
A novel rich source of human mesenchymal stem cells from the debris of bone marrow samples
Section snippets
Materials and methods
Isolation and culture of MSC form BM debris and BM. BM was harvested from 7 donors (three women and four men at the age ranging from 28 to 58 years,) following informed consent according to the guideline from Beijing Institute of Radiation Medicine Committee on the Use of Human Subjects in Research. Collected marrow was passed through a cell-strainer with the pore size of 80 μm in diameter (BD, USA). Mononuclear cells were isolated from the filtered BM by the routine density gradient
Results
To observe if MSCs existed in the debris of human BM samples, the tissue fragments were digested with collagenase and mononuclear cells were harvested by gradient centrifugation. The proportion of mononuclear cells was about 80% in the cell suspension from the digested BM debris, which was obviously higher than that in the BM analogue. Moreover, typically, the number of mononuclear cells in the digested debris could reach up to half of that from the filtered marrow.
When the digested cells were
Discussion
Although MSCs can be isolated from many tissues, bone marrow is a reproducible and convenient source of these cells from nearly all species tested except mice [11]. In this study, we tried to isolate MSCs from the tissue fragments in the human marrow samples, which are mainly composed of adipose tissue, compact bone fragments and vessel networks. Our results here suggest that these cells meet the generally accepted standard criteria including the fibroblast-like morphology, the expression of
Acknowledgments
This research was supported by Chinese High-tech Grant (No. 2007AA02Z454) and Beijing Medical Development Grants (No. 2007-2033 and No. 2006-2042).
References (21)
- et al.
Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells
J. Orthop. Res.
(2002) - et al.
Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells
Biochem. Biophys. Res. Commun.
(2006) - et al.
Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts
Exp. Hematol.
(2008) - et al.
Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood
Exp. Hematol.
(2005) - et al.
Induction of neural-like differentiation in human mesenchymal stem cells derived from bone marrow, fat, spleen and thymus
Bone
(2007) - et al.
Role of mesenchymal stem cells in regenerative medicine: application to bone and cartilage repair
Expert Opin. Biol. Ther.
(2008) - et al.
Mesenchymal stem cell-based repair of articular cartilage with polyglycolic acid-hydroxyapatite biphasic scaffold
Int. J. Artif. Org.
(2008) - et al.
Potential of bone marrow stromal cells in applications for neuro-degenerative, neuro-traumatic and muscle degenerative diseases
Curr. Neuropharmacol.
(2005) - et al.
Immunomodulation by mesenchymal stem cells: a potential therapeutic strategy for type I diabetes
Diabetes
(2008) - et al.
Mesenchymal stem cells for treatment of therapy-resistant graft-versus-host disease
Transplantation
(2006)
Cited by (13)
Secreted galectin-3 as a possible biomarker for the immunomodulatory potential of human umbilical cord mesenchymal stromal cells
2013, CytotherapyCitation Excerpt :However, whether the aforementioned factors represent the only mediators in the suppression of T-cell function is not evident, and blocking any single one of these molecules did not completely abrogate the immunosuppressive functions of MSCs. MSCs have been identified and isolated from different tissue sources, such as bone marrow (BM-MSCs), umbilical cord (UC-MSCs) and adipose tissue (20–22). Compared with MSCs isolated from other sources, UC-MSCs have distinct advantages, including more primitive properties, easy accessibility, higher proliferation capacity and lower immunogenicity.
The aggregate nature of human mesenchymal stromal cells in native bone marrow
2012, CytotherapyCitation Excerpt :Consistent with our findings, Jin et al. (26) have suggested previously that ‘BM debris’ not filtered through a cell strainer might serve as a new source of MSC, and Dvorakova et al. (27) observed that substantial amounts of MSC were entrapped in BM collection bags. We believe that BM debris, as named by Jin et al. (26), is another description of what we have called here MSC-rich aggregates. It is noteworthy that some of the MSC-rich aggregates isolated by the filtration method contained fat droplets.
Challenges in Mesenchymal Stromal Cell-based Therapies
2023, Current Stem Cell Research and Therapy