Curcumin-induced histone hypoacetylation: The role of reactive oxygen species
Section snippets
Materials
Curcumin (Cur), RPMI-1640 medium, trichostatin A (TSA), superoxide dismutase (SOD) and catalase (CAT) were purchased from Sigma. The antibodies recognized histone H3 or H4 acetylated at their N-terminal lysine residues were purchased from Upstate Biotechnology. [3H]-acetate and [3H]-acetyl-CoA were purchased from Amersham. The BCA protein assay kit was purchased from Pierce.
Cell culture and treatment
Human hepatoma Hep3B cells were grown in RPMI-1640 medium with 10% FCS, antibiotics and 5% CO2 at 37 °C. After culturing
Inhibition of Cur on histone acetylation in Hep3B cells
Firstly, effect of Cur on histone acetylation was detected by assaying the incorporation of [3H] acetate in histones in Hep3B cells. At low concentrations (on more than 20 μM), Cur treatment led to no obvious alteration of histone acetylation, while at high concentrations (no less than 25 μM), Cur resulted in a concentration- and time-dependent decrease in histone acetylation (Fig. 1A and B). The inhibition of histone acetylation was sustained for all tested times (24 h). This inhibitory effect of
Discussion
Curcumin is a well-known dietary pigment derived from the plant Curcuma longa. Previous studies indicated that it efficiently induces the proliferation arrest and cell death (including apoptosis and necrosis) in a variety of tumor cells [11], [12], [13], [14], [15], [16], but its in vivo target molecules and anticancer mechanisms remain to be clarified. The present study suggests that inhibition of histone acetylation was one new mechanism for the anticancer activity of Cur, where HAT serves as
Acknowledgements
This project was supported by the National Natural Science Foundation of China, 30400230, the Grant of Lanzhou University, 5715220101, and that from SRF for ROCS, SEM.
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