Original articlesAntipsychotic drugs elevate mRNA levels of presynaptic proteins in the frontal cortex of the rat
Section snippets
Animals
Male Sprague-Dawley rats (Taconic Farms, Germantown, New York) weighing 200 g at the beginning of the experiment were housed four to a cage on a 12-hour light-dark cycle. The animals were allowed 1 week for habituation to the colony before the first drug administration. All rats received single, daily injections, administered intraperitoneally (IP). Animal care and experimental procedures conformed to PHS Policy on Humane Care and Use of Laboratory Animals.
Results
A triple data analysis was performed with dChip, MAS 5.0 and RMAExpress. Most differences in expression levels of abundantly expressed genes treated with APDs and analyzed with dChip 1.3 were below twofold. Genes with lower expression levels and high signal-to-noise ratios yielded higher-fold differences. For example, syntaxin 12 was expressed eightfold higher in the FC of APD treated rats than in vehicle-treated control subjects but was above detection level in only 9 of 28 samples (32%
Discussion
Changes in mRNA levels have been previously correlated with similar changes in protein levels. For example, acute treatment with haloperidol caused upregulations of c-fos, proenkephalin, and synapsin II mRNAs that translated into upregulations of these proteins in the striatum (Chong et al 2002; Dragunow et al 1990; Hong et al 1979, 1985; Konradi and Heckers 1995; Konradi et al 1993; Leveque et al 2000). Although these findings are encouraging for microarray experiments, it is important to keep
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