Original ArticleThe impact of arginine on bacterial translocation in an intestinal obstruction model in rats
Section snippets
Background
Nutrition has usually been seen as the supply of energy and nitrogen to maintain body mass. Recent studies have, however, revealed that nutrition also plays an important role in modulating the immune and inflammatory responses. The clinical relevance of this modulation has led to the investigation of diets containing specific substrates the so-called immunonutrients, such as glutamine, arginine, nucleotides and omega-3 fatty acids.1 These formulas have been related to modulation of gut
Animals
Forty male Wistar rats weighting between 250 and 350 g were included in this study. The animals were randomized to five groups of eight animals each one: (1) “sham” operated (no obstruction; inoculation of 12.5 MBq of 99mTechnetium-Escherichia coli (99mTc-E. coli)) fed a conventional chow diet (LABINA®, Purina, Brazil; 23% protein); (2) IO group fed a conventional chow diet, submitted to intestinal obstruction and inoculation of 12.5 MBq of 99mTc-E. coli; (3) Arginine 300 mg group fed conventional
Composition of standard chow and supplemented chows
The composition of standard and supplemented chows is shown in Table 1. There was an increase in total calories in the Impact formula (355.6 kcal/100 g) compared to the conventional chow (275 kcal/100 g) and the arginine supplemented chow (264.3 kcal/100 g).
There was an increase of protein, nitrogen and arginine in the chow supplemented with arginine and an increase of arginine, fat and total calories in the chow supplemented with the enteral formula. Carbohydrate content was slight reduced in all
Discussion
The role of arginine in preventing bacterial translocation in an experimental intestinal obstruction model was assessed. Although arginine has been reported to reduce bacterial translocation in experimental obstructive jaundice models by recovering viable organisms from the mesenteric lymph node (MLN) and spleen16, 17, 18 in our study we used 99mTechnetium E. coli. This method is very fast, direct, simple and does not require aseptic conditions throughout the experiments.13, 15 Another key
Acknowledgments
Ajionomoto, Brazil and Novartis Brazil for having, respectively, given Arginine and the Impact formula. CNPQ (National Counsel of Technological and Scientific Development), Fundep (Fundação de Desenvolvimento da Pesquisa) for the grants.
Iara Eliza Pacífico Quirino contributed the conception and design of the experiment and experimental procedures, statistical analysis and interpretation of data; Maria Isabel T.D. Correia the conception and design of the study, interpretation of the data,
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