Antimicrobial Susceptibility Studies
Evaluation of apramycin activity against carbapenem-resistant and -susceptible strains of Enterobacteriaceae

https://doi.org/10.1016/j.diagmicrobio.2016.09.002Get rights and content

Highlights

  • Over 70% of carbapenem-resistant Enterobacteriaceae are susceptible to apramycin

  • Activity spectrum is broader than or equal to currently used aminoglycosides

  • Apramycin modifying enzymes are rare among human Enterobacteriaceae isolates

Abstract

We evaluated activity of apramycin, a non-ototoxic/non-nephrotoxic aminocyclitol against 141 clinical Enterobacteriaceae isolates, 51% of which were non-susceptible to carbapenems (CRE). Among CRE, 70.8% were apramycin susceptible, which compared favorably to aminoglycosides in current clinical use. Our data suggest that apramycin deserves further investigation as a repurposed, anti-CRE therapeutic.

Section snippets

Funding Information

This work was supported in part by a Chief Academic Officer's Pilot Grant from Beth Israel Deaconess Medical Center.

Acknowledgements

We thank Thea Brennan-Krohn, Lucius Chiaraviglio, and Jennifer Tsang for critical reading of the manuscript.

References (32)

  • Clinical and Laboratory Standards Institute (CLSI)

    Performance standards for antimicrobial susceptibility testing; twenty-sixth informational supplement

  • J Davies et al.

    Enzymatic modification of aminoglycoside antibiotics: 3-N-acetyltransferase with broad specificity that determines resistance to the novel aminoglycoside apramycin

    Antimicrob Agents Chemother

    (1978)
  • J Davies et al.

    Inhibition of protein synthesis by spectinomycin

    Science

    (1965)
  • Y Doi et al.

    16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosides

    Clin Infect Dis

    (2007)
  • AM Haritova et al.

    Pharmacokinetics of gentamicin and apramycin in turkeys roosters and hens in the context of pharmacokinetic-pharmacodynamic relationships

    J Vet Pharmacol Ther

    (2004)
  • EB Hirsch et al.

    Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging cause of multidrug-resistant infection

    J Antimicrob Chemother

    (2010)
  • Cited by (0)

    View full text