Elsevier

Fertility and Sterility

Volume 106, Issue 2, August 2016, Pages 402-409
Fertility and Sterility

Original article
Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis

https://doi.org/10.1016/j.fertnstert.2016.04.013Get rights and content
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Objective

To investigate serum microRNAs (miRNAs) in women with endometriosis.

Design

Case-control study.

Setting

University hospital.

Patient(s)

Women with (n = 24) and without (n = 24) endometriosis.

Intervention(s)

Serum samples were obtained from surgically diagnosed subjects.

Main Outcome Measure(s)

miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs.

Result(s)

miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150–5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model.

Conclusion(s)

We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.

Key Words

Endometriosis
biomarker
miRNA
serum
microarray

Cited by (0)

E.C. has nothing to disclose. R.M. has nothing to disclose. G.S.E. has nothing to disclose. S.C. has nothing to disclose. B.S. has nothing to disclose. H.S.T. has nothing to disclose.

Supported by a grant from Ovascience, Boston, Massachusetts.