Short reportCytotoxic activity screening of some indigenous Thai plants
Section snippets
Plants
Fourteen plants, listed in Table 1 were collected during 2002 to 2003 from Khon Kaen Thailand, where is a sanctuary area for Plant Genetics Conservation Project under the Royal Initiation of her Royal Highness Princess Maha Chakri Sirindhorn. Voucher specimens were deposited in the Center for Research and Development of Herbal Health Products (CRD-HHP), Khon Kaen University, Thailand.
Uses in traditional medicine
See Table 1.
Previously isolated class of constituents
See Table 1.
Tested material
50% Ethanol extracts (yields in Table 2). Stock samples of each extract were dissolved in dimethyl sulfoxide at a concentration of 100 mg/ml.
Cytotoxic activity
The stock samples were diluted with Dulbecco's Modified Eagle's Medium (DMEM) to desired concentrations ranging from 62.5 to 1000 µg/ml. The final concentration of DMSO in each sample did not exceed 1%v/v. The cytotoxic activity of the extracts were tested in malignant human hepatoma (HepG2) cell line and normal African green monkey kidney (Vero) cell line by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method [26] with minor modification. Melphalan was used as a
Results and conclusions
Cytotoxicity results are summarized in Table 2. In consideration of the cytotoxicity and selectivity result, the samples could be classified into 5 categories. Firstly, potentially cytotoxic (IC50 in HepG2 < 100 µg/ml) and high selectivity (SI ≥ 3) are melphalan, P. evecta and E. cuneatum. Secondly, moderate cytotoxic (100 µg/ml < IC50 in HepG2 < 1000 µg/ml) and high selectivity (SI ≥ 3) are P. debilis, C. filiformis and G. cowa. Thirdly, moderate cytotoxic (100 µg/ml < IC50 in HepG2 ≤ 1000 µg/ml) but less
Acknowledgements
Pokpong Prayong is grateful for the Graduate School, Khon Kaen University for the financial support (Grant No. 50111113). The authors thank to the Plant Genetics Conservation Project under the Royal Initiation of her Royal Highness Princess Maha Chakri Sirindhorn for permission of conducting the research and to Dr. Chanvit Leelayuwat, Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Khon Kaen University for providing the cell lines.
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