Original ContributionIncreasing extracellular H2O2 produces a bi-phasic response in intracellular H2O2, with peroxiredoxin hyperoxidation only triggered once the cellular H2O2-buffering capacity is overwhelmed
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Graphical abstract
Abbreviations
Prx
peroxiredoxins
Trx
thioredoxin
Txl1
thioredoxin-like protein 1
Gpx1
glutathione peroxidase 1
AMS
4-acetamido-4ʹ-((iodoacetyl)amino)stilbene-2,2ʹ-disulfonic acid
NEM
N-ethylmaleimide
ROS
reactive oxygen species
H2O2
hydrogen peroxide
CysP
peroxidatic cysteine
CysR
resolving cysteine
Pr-SS
protein disulfides
Pr-SH
protein thiols
DMEM
Dulbecco’s Modified Eagles Medium
EMM
Edinburgh minimal media
TCA
trichloroacetic acid
DPBS
Dulbecco’s phosphate-buffered saline
HRP
horse radish peroxidase
PF3
acetylated peroxyfluorescein 3
AIC
Akaike Information Criterion
Keywords
Peroxiredoxin
Hydrogen peroxide
Thiol
Computational model
Signaling
Oxidation
Thioredoxin
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Current Address: School of Health, Sport and Biosciences, University of East London, London E15 4LZ, UK.
© 2016 The Authors. Published by Elsevier Inc.