Genetic polymorphisms of 22 autosomal STR loci in Chinese Han population

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Abstract

Microreader™ 23sp ID system was a new STR multiplex system developed for forensic application, but there was a lack of population data in the Chinese Han population. This kit contained 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290, D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus. In this study, a population sample in Chengdu Han population was analyzed with the kit. Forensic genetic parameters and probability values of the Hardy–Weinberg equilibrium (HWE) were calculated with software PowerStats v1.2 and Arlequin v3.5. Allele frequencies of the 22 STR loci and further forensic genetic parameters were obtained. The results suggested that Microreader™ 23sp ID system can provide informative polymorphic data for identification and parentage testing in Chinese Han population.

Introduction

STRs was widely used in paternity test, individual identification and biological anthropology because they can be easily amplified by the polymerase chain reaction (PCR) [1], and its diversity and genetic differentiation of human populations. Nevertheless, there was an absence of genetic information for some countries or areas. In this study, 22 autosomal STR loci in the Chengdu Han population were investigated using the Microreader™ 23sp ID system, to obtain the data of population genetics and determine the genetic variability.

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Materials and methods

Blood samples were collected from 152 unrelated individuals (109 males and 43 females) of the Han population living in Chengdu, northwest China after informed consent. Genomic DNA was isolated by the salting out extraction method and quantified by the NanoDrop1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). The samples were amplified using the Microreader™ 23sp ID system (Suzhou Microread Genetics, Suzhou, Jiangsu, China), PCR amplification was carried out on a Mastercycler®

Results and discussion

Allele frequencies and forensic parameters were listed in Table 1. The observed heterozygosity (Ho) ranged from 0.7171 (D10S1435) to 0.8684 (D2S1338) while the expected heterozygosity (He) ranged from 0.7294 (D10S1435) to 0.8733 (D7S3048). The power of discrimination (PD) ranged from 0.8831 (D10S1435) to 0.9627 (D11S2368) and the probability of excluding paternity (PE) ranged from 0.4552 (D10S1435) to 0.7315 (D2S1338). The combined power of discrimination (CPD) in the 22 STR loci was

Conclusion

Our results showed that Microreader™ 23sp ID system could amplify 22 autosomal STR loci (contained 21 non-CODIS STR loci) and one sex-determining locus in a single reaction and it presented extremely high CPD and CPE value in the Chengdu Han population. Based on these we suggest that Microreader™ 23sp ID system could provide informative polymorphic data for identification and parentage testing in Chinese Han population, especially for deficiency cases of paternity test and the presence of STR

Conflict of interest

None.

Acknowledgements

The authors would like to thank all volunteers who donated samples. This work was supported by the National Natural Science Foundation of China (81273349, 81330073).

References (3)

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There are more references available in the full text version of this article.

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Both authors contributed equally to this work.

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