Research PaperClinical PathologyHigh-dose zoledronic acid narrows the periodontal space in rats
Section snippets
Materials and methods
40 male Wistar rats (Nihon SLC, Shizuoka, Japan: body weight 300–350 g; 10–12 weeks old) were used in the experiment. All rats were housed in cages with free access to food and water, and a 12 h light/dark cycle was maintained. All experiments were approved and performed in accordance with the guidelines for Animal Experiments Ethic Committee of Yokohama City University.
The 40 rats were randomly divided into four groups. Groups A, B, and C received ZA at doses of 10, 50, and 500 μg/kg,
Results
The experiment was performed without any complications, and no infection was observed in any of the rats. Histological examination revealed that neither spontaneous soft tissue necrosis nor spontaneous ONJ was observed in any of the rats.
The cross-sectional area of the incisor tended to narrow in a ZA dose-dependent manner. The narrowest and widest mean values and the standard deviations in the short-term groups were 2.340 ± 0.067 (group Cs) and 2.384 ± 0.117 mm2 (group Ctls), respectively, whereas
Discussion
Local inflammation and connective soft tissue reactions to infection are observed in most cases of ONJ.14, 18 Stephen et al. reported that the administration of ZA with dexamethasone prior to dental extractions in rats resulted in the development of histopathological changes that were similar to ONJ in humans.13 These findings indicate that ONJ occurs in the presence of any factor associated with bacterial infection of an intraoral wound. In the present study, the authors examined the effects
Funding
This study was supported by Novartis Pharma AG and in part by a Grant-in-Aid for Young Scientists (B) (No. 23792366) from the Japan Society for the Promotion of Science.
Competing interests
None declared.
Ethical approval
Animal experiments were approved by the ethics committee at Yokohama City University (No. 10-027).
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