Mechanisms of asthma and allergic inflammationGene expression profiles in human nasal polyp tissues studied by means of DNA microarray
Section snippets
Subjects
All studies were conducted by using a human subject research protocol approved by Johns Hopkins Medicine Institutional Review Board.
NP tissues for DNA microarray analysis were obtained from 10 patients with CRS (8 women) between the ages of 27 and 67 years (mean, 51 years). Of these, 3 were identified as allergic on the basis of positive puncture skin test results to a standard panel of aeroallergens. Five of the 10 patients had physician-diagnosed asthma. Two of 10 patients were aspirin
Transcriptional profile in NP
The genes meeting the following criteria were admitted to the list of genes differentially expressed in patients with NP compared with those in healthy patients to ensure a minimal number of false-positive results: (1) the difference of gene expression level between NP and normal tissues was statistically significant, with a P value of less than .05; (2) the expression difference was larger than 2-fold; (3) genes could be called present according to the absolute call criteria in at least half
Discussion
DNA microarrays were used to test more than 10,000 human genes to screen for those in which expression was altered at least 2-fold in NP tissue compared with normal sinus mucosa. This strategy identified 192 upregulated and 156 downregulated genes (Appendixes 4 and 5 in the Journal's Online Repository). The utility of this approach to provide insight into disease pathogenesis is highlighted by the fact that although a few of these genes have been previously implicated in the pathogenesis of NP,
References (42)
- et al.
Nasal polyps in asthma and rhinitis. A review of 6,037 patients
J Allergy Clin Immunol
(1977) - et al.
Nasal polyposis: immunohistochemistry and bioelectrical findings (a hypothesis for the development of nasal polyps)
J Allergy Clin Immunol
(1997) - et al.
Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps
J Allergy Clin Immunol
(2000) - et al.
Distinct transcriptional profiles of adrenocortical tumors uncovered by DNA microarray analysis
Am J Pathol
(2003) - et al.
Nasal mucosal gene expression in patients with allergic rhinitis with and without nasal polyps
J Allergy Clin Immunol
(2003) - et al.
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method
Methods
(2001) - et al.
A novel aspartyl proteinase from apocrine epithelia and breast tumors
J Biol Chem
(2000) - et al.
Bacteriostatic activity of human lactoferrin against Staphylococcus aureus is a function of its iron-binding properties and is not influenced by antibiotic resistance
FEMS Immunol Med Microbiol
(2001) - et al.
Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily
J Biol Chem
(2002) - et al.
Detection of the chemokine RANTES and endothelial adhesion molecules in nasal polyps
J Allergy Clin Immunol
(1996)
Effects of topical glucocorticoids on in vitro lactoferrin glandular secretion: comparison between human upper and lower airways
J Allergy Clin Immunol
Prolactin-inducible proteins in human breast cancer cells
J Biol Chem
Nasal polyps: relationship to infection and inflammation
Nasal polyposis: an update. Editorial review
Curr Opin Allergy Clin Immunol
A histological study of formation and growth of nasal polyps
Acta Otolaryngol
Microarray analysis of eosinophils reveals a number of candidate survival and apoptosis genes
Am J Respir Cell Mol Biol
Gene expression profiling of 12633 genes in Alzheimer hippocampal CA1: transcription and neurotrophic factor down-regulation and up-regulation of apoptotic and pro-inflammatory signaling
J Neurosci Res
Evaluation of normalization procedures for oligonucleotide array data based on spiked cRNA controls
Genome Biol
Matrix metalloproteinases MMP-7, MMP-9 and their tissue inhibitor TIMP-1: expression in chronic sinusitis vs nasal polyposis
Allergy
Analysis of the cellular infiltrate and epithelial class I and II molecular expression in edematous type nasal polyps
Acta Otorhinolaryngol Ital
Structural features of salivary function
Crit Rev Oral Biol Med
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Dr Liu is currently affiliated with the Department of ENT, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Dr Wang is currently affiliated with Merck Research Laboratory, Molecular Profiling, Seattle, Wash.
Supported by NIH/NIDCR grants DE07652, DE05672, and DE14950 (to F.G.O.).
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Drs Liu and Kim contributed equally to this article.