Food allergy, dermatologic diseases, and anaphylaxisAbsence of T-regulatory cell expression and function in atopic dermatitis skin
Section snippets
Subjects
PBMCs were isolated from peripheral blood of 15 healthy volunteers or patients with AD (aged 19-45 years) hypersensitive to house dust mite (HDM) or birch pollen allergens and then purified or cultured to provide the various types of T cells used in this study.
Twenty-four–hour positive atopy patch test (APT) biopsies were taken of 3 patients with AD at the University Medical Center Utrecht, The Netherlands, as previously described.15 Three psoriasis biopsies were obtained from the ZAUM-Center
Expression of IL-10 and TGF-β as well as their receptors, but not FoxP3, in lesional AD skin
To investigate the expression and function of Treg cells in AD, we looked at whether the Tr1 cell-specific cytokines, IL-10 and TGF-β, or CD4+CD25+ Treg cell-specific transcription factor FoxP3 are expressed in lesional AD skin. Despite the presence of large numbers of CD25+ cells (Fig 1, A), we did not detect any FoxP3+ cells, neither in the dermal infiltrate of chronic lesional AD skin nor in acutely inflamed skin 24 hours after APT, psoriatic skin, or healthy skin (Fig 1, B). FoxP3 was
Discussion
During the last decade, a significant amount of data has accumulated on the suppressive effects of Tr1 or CD4+CD25+ T-regulatory cells in models of autoimmunity, allergy, transplantation tolerance, tumor tolerance, and chronic infections.12, 23 The efficacy of various Treg cell subsets in the suppression of inflammation has tempted scientists to speculate that increasing Treg cell numbers may suppress inflammation and tissue injury in affected organs. In the current study, we show that FoxP3+
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Authors' laboratories supported by Swiss National Science Foundation grants No. 31-105865 and 32-100266 and the Global Allergy and Asthma European Network (GA2LEN).