Environmental and occupational respiratory disordersLimited IgE cross-reactivity between Dermatophagoides pteronyssinus and Glycyphagus domesticus in patients naturally exposed to both mite species
Section snippets
Patients
Consecutive patients (n = 136) from rural and urban areas in the province of Huelva (southwest Spain) were selected. The patients had a positive skin prick test (SPT) response to Dermatophagoides pteronyssinus (ALK-Abelló, S.A. Madrid, Spain) and were given diagnoses of rhinoconjunctivitis, asthma, or both caused by sensitization to mites. Each patient's house dust sample was taken and processed as indicated. All subjects provided written informed consent.
House dust samples
House dust samples were collected in
Results
A total of 136 house dust samples from patients sensitized to D pteronyssinus were analyzed, and the following mite species were identified: D pteronyssinus in 129 (94.8%) samples, D farinae in 30 (22.0%) samples, G domesticus in 74 (54.4%) samples, T putrescentiae in 56 (41.1%) samples, L destructor in 23 (16.9%) samples, Acarus siro in 15 (11%) samples, and B tropicalis in 9 (6.6%) samples. D pteronyssinus was the most abundant mite, both in terms of frequency and relative amount, with a
Discussion
In our study D pteronyssinus was the most frequent mite species, present in 94.8% of the house dust samples from patients with mite allergy. A similar prevalence was found in samples obtained from the general population.15 In addition, it was the most abundant mite, representing, on average, about 43% of the mite count. D farinae was present in only 22% of samples and always in a low amount, which is in line with the results of a previous study.15 G domesticus was the second most abundant mite
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Disclosure of potential conflict of interest: M. Lombardero, C. Arteaga, J. A. Carpizo, and D. Barber are employed by ALK-Abelló. J. Arias-Irigoyen has declared that he has no conflict of interest.