Clinical researchDetection and Eradication of Microorganisms in Root-filled Teeth Associated With Periradicular Lesions: An In Vivo Study
Section snippets
Clinical Material
Twenty patients (10 women and 10 men; age, 25 to 75 years; mean age, 47 years) who were referred for endodontic treatment to the University Clinic and Dental Hospital, Freiburg, Germany, were selected for this study. The patients were not enrolled into the study if any of the following criteria were present: (1) severe systemic diseases, (2) pregnancy or lactation, (3) use of any antibiotics within the past 30 days, (4) participation in another clinical study during the previous 3 months, (5)
Microbial Cultures
With regard to the cultures obtained after removal of the root canal filling material (S0), 12 teeth (60%) contained cultivable microorganisms. Of these 12 teeth, the quantity of microorganisms recovered ranged from 0 to 7.8 × 107 CFU/mL (with a median of 3.5 × 103 CFU/mL) and from 3.0 × 103 to 1.0 × 109 CFU/mL (with a median of 1.5 × 105 CFU/mL) with regard to aerobic and anaerobic cultivation, respectively. After irrigation with EDTA and NaOCl (S1) and after irrigation with CHX (S2), none of
Discussion
Culture samples showed that 60% of the root-filled teeth associated with periradicular disease yielded microorganisms. Using PCR, in 65% (13/20) of the teeth bacterial DNA was detected. In four of those samples (31%), DNA of E faecalis was found. After further root canal preparation and irrigation using NaOCl and EDTA, microorganisms could be detected in none of the teeth. Thus, CHX and the intracanal dressing with Ca(OH)2 could not show further disinfection. In contrast, microorganisms were
Acknowledgments
The authors express their sincere appreciation to Dr. K. Pelz for his helpful criticism and suggestions.
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