Clinical ResearchAnalysis of Symptomatic and Asymptomatic Primary Root Canal Infections in Adult Norwegian Patients
Section snippets
Subjects, Sample Taking, and DNA Extraction
Root canal samples were taken from 43 Norwegian patients presenting to an endodontic private practice office in Bekkestua, Norway for treatment of teeth with apical periodontitis. Teeth included in this study were from adult patients, all of them with necrotic pulps and radiographic evidence of apical periodontitis. Each patient contributed one tooth. Twenty-one cases showed no pain or sinus tract at the time of treatment and were diagnosed as asymptomatic apical periodontitis. Nine cases
Results
All sample extracts were positive for PCR amplification by using the universal 16S rRNA gene primers, indicating that bacterial DNA was present in all examined samples and was available for analyses, and significant inhibitors of the PCR reaction were not present. Negative controls yielded no amplicons.
Overall, the results of the reverse-capture checkerboard analysis revealed that 48 of the 50 oligonucleotide probes tested were reactive with 1 or more clinical samples. In general, the number of
Discussion
The desire to find a single or at least a group of major species that is associated with acute symptoms is an ever recurrent theme in the study of endodontic infections. Nonetheless, evidence accumulated over the years from studies with diverse methodologies has suggested that there might be neither a single nor even a small group of bacterial culprits when it comes to endodontic symptomatology (10). Endodontic infections are usually characterized by multispecies biofilm communities (11), and
Acknowledgments
The authors are grateful to Mr Marlei Gomes da Silva for his valuable technical assistance.
The authors deny any conflicts of interest related to this study.
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2019, AnaerobeCitation Excerpt :In the study of Socransky et al., P. micra was placed in the orange complex along with Prevotella intermedia, Prevotella nigrescens and F. nucleatum indicating that it is often found at periodontitis sites [11]. In addition, it is associated with apical periodontitis lesions [38,39] and necrotic root canals [40]. Viable bacteria have also been isolated from the bloodstream following dental procedures and periodontal therapy and have been implicated in infections at many different sites in the body, including chronic skin infections, destructive knee joint infections prosthetic hip joint infections and soft tissue infections at remote sites [for a review see 41].
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This study was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazilian Governmental Institutions.