Elsevier

Journal of Endodontics

Volume 38, Issue 9, September 2012, Pages 1239-1243
Journal of Endodontics

Basic Research
Decalcifying Effect of Different Ethylenediaminetetraacetic Acid Irrigating Solutions and Tetraclean on Root Canal Dentin

https://doi.org/10.1016/j.joen.2012.06.010Get rights and content

Abstract

Introduction

The objective of this study was to evaluate and compare in vitro the decalcifying capability on root canal dentin of 4 different irrigating solutions.

Methods

Twenty-five freshly extracted maxillary central incisors were selected. The canals were prepared to obtain a total of 4 samples from each root. The samples were randomly divided into 5 equal experimental groups (n = 20) according to the irrigating solutions tested (Tetraclean, Largal Ultra, 17% ethylenediaminetetraacetic acid, Tubuliclean). For each irrigating solution, the specimens were immersed for 5, 10, and 15 minutes. After exposures, the concentration of Ca2+ extracted was measured by inductively coupled plasma-atomic emission spectrometry. Data were analyzed by means of nonparametric tests, and statistical analysis was performed.

Results

The higher release of Ca2+ was observed in samples treated with Tetraclean; this citric acid–based agent showed significantly higher decalcifying capability. For all irrigating solutions tested, 10 minutes of application are sufficient to obtain the maximum Ca2+ release.

Conclusions

The results indicated that Tetraclean could be applied to obtain more efficient removal of the smear layer and to facilitate the biomechanical instrumentation procedures.

Section snippets

Materials and Methods

Twenty-five human maxillary central incisors, freshly extracted for periodontal reasons, were stored in 0.1% thymol solution until use. All samples were intact teeth with vital pulp extracted in patients of similar age (mean age, 56 ± 5 years). The crowns were removed at cementoenamel junction by using an Accutom-50 diamond cutter (Accutom Hard Tissue Microtome; Struers, Ballerup, Denmark) under water-cooling. The root surface was treated with a low-speed fine-grain diamond bur (Perio-Set;

Results

The efficacy of 4 commercial irrigating solutions used to remove calcium from root dentin has been examined. The release at different equilibration times was considered; in particular, the dentin specimens was contacted with each agent for 5, 10, and 15 minutes. Descriptive statistics of the values of Ca2+ released from root dentin at 5, 10, and 15 minutes have been calculated. Kruskal–Wallis showed significant differences among the various groups (P < .001). Mann–Whitney U test was applied for

Discussion

The null hypothesis of the study has been rejected. The higher release of Ca2+ was observed in samples submitted to citric acid–based agent; Tetraclean showed greater decalcifying capability, significantly higher than other tested EDTA-based agents. The amount of Ca2+ extracted from root dentin samples for all irrigating solutions at 10 minutes did not show significant differences when compared with the values obtained at 15 minutes (P > .05). This means that in the present investigation, 10

Conclusions

Under the experimental conditions and restricting to the irrigating solutions considered in the present investigation, the results indicated that the higher release of Ca2+ observed in samples treated with Tetraclean demonstrated its significantly higher decalcifying capability than other irrigants tested. Therefore, Tetraclean can be applied to obtain an efficient removal of the smear layer and to facilitate the biomechanical instrumentation procedures.

Further investigations are requested to

Acknowledgments

The authors deny any conflicts of interest related to this study.

References (34)

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