In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material

doi:10.1016/j.joen.2012.11.026

Journal of Endodontics

Volume 39, Issue 4, April 2013, Pages 478-483

https://doi.org/10.1016/j.joen.2012.11.026 Get rights and content

Abstract

Introduction

This study examined the effect of a new bioactive dentin substitute material (Biodentine) on the viability of human gingival fibroblasts.

Methods

Biodentine, White ProRoot mineral trioxide aggregate (MTA), and glass ionomer cement were evaluated. Human gingival fibroblasts were incubated for 1, 3, and 7 days both in the extracts from immersion of set materials in culture medium and directly on the surface of the set materials immersed in culture medium. Fibroblasts cultured in Dulbecco modified Eagle medium were used as a control group. Cytotoxicity was evaluated by flow cytometry, and the adhesion of human gingival fibroblasts to the surface of the set materials was assessed by using scanning electron microscopy. The data of cell cytotoxicity were analyzed statistically by using a one-way analysis of variance test at a significance level of P < .05.

Results

Cells exposed to extracts from Biodentine and MTA showed the highest viabilities at all extract concentrations, whereas cells exposed to glass ionomer cement extracts displayed the lowest viabilities (P < .05). There was no significant difference in cell viabilities between Biodentine and MTA during the entire experimental period (P > .05). Human gingival fibroblasts in contact with Biodentine and MTA attached to and spread over the material surface after an overnight culture and increased in numbers after 3 and 7 days of culture.

Conclusions

Biodentine caused gingival fibroblast reaction similar to that by MTA. Both materials were less cytotoxic than glass ionomer cement.

Section snippets

Materials and Methods

Two commercially available calcium silicate–based cements, Biodentine (batch #B01647) and White ProRoot MTA (batch #11004158), and a glass ionomer cement (GIC) (GC Fuji IX GP, batch #1111021; GC Corporation, Tokyo, Japan) were used in the present study.

Cytotoxicity of the Set Cements

Results of the flow cytometry assay on the cell viability of fibroblasts in the different concentrations of extracts derived from the set calcium silicate–based (Biodentine and MTA) and GIC materials are summarized in Figure 1. Cell viability depended on the type of material, culture medium, and incubation time that the cells were exposed to. After culturing for 1 day, cells incubated with extracts from Biodentine and MTA showed the highest viabilities at all extract concentrations, whereas

Discussion

Materials used in endodontics should preferably be biocompatible. This is particularly desirable when they are placed in direct contact with living tissue such as in pulp capping, perforation repair, or when used as a retrograde filling. Biodentine, a new calcium silicate–based material, is designed to be placed in permanent and close contact with periradicular tissue, and therefore, it is important to assess its possible cytotoxic effects on human gingival fibroblasts. MTA, a calcium

Conclusions

Human gingival fibroblasts showed similar response to extracts from Biodentine and MTA as measured by cytotoxicity assay and cell growth on set materials.

Acknowledgments

The authors deny any conflicts of interest related to this study.

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: Supported by the Natural Science Foundation of Heilongjiang Province of China (no. ZD201012).

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Basic ResearchIn Vitro Cytotoxicity Evaluation of a Novel Root Repair Material

Abstract

Introduction

Methods

Results

Conclusions

Section snippets

Materials and Methods

Cytotoxicity of the Set Cements

Discussion

Conclusions

Acknowledgments

Dent Mater

J Endod

J Endod

J Endod

J Endod

Dent Mater

Dent Mater

J Endod

J Endod

J Endod

J Endod

J Endod

J Endod

J Endod

J Endod

J Endod

Oral Surg Oral Med Oral Pathol Oral Radiol Endod

J Endod

J Immunol Methods

Basic Research
In Vitro Cytotoxicity Evaluation of a Novel Root Repair Material