Basic ResearchRosiglitazone Inhibits Proliferation and Induces Osteopontin Gene Expression in Human Dental Pulp Cells
Section snippets
Human Dental Pulp Cell Isolation and Culture
This study was previously approved by the Ethics Board of the School of Health Science at the University of Brasilia, and informed consent was obtained from all participants. The hDPCs were cultured by using the explant technique (12). Briefly, pulp tissues were obtained from non-erupted, caries-free third molars of young and healthy patients. The tissues were minced into small fragments and placed into 35-mm culture dishes with high-glucose Dulbecco modified Eagle medium (DMEM) supplemented
Rosiglitazone Reduces hDPC Proliferation
RSG (10 μmol/L) inhibited hDPC proliferation after 24 hours by 57.2% ± 4.097% (P < .001), compared with vehicle-treated cells (Fig. 1A). Cell viability was not affected by RSG treatment (Fig. 1B), which also did not induce apoptosis or necrosis of hDPCs (Fig. 1C). Moreover, RSG (10 μmol/L) did not impair hDPC migration ability during the 48-hour period after scratches were made (Fig. 2).
Rosiglitazone Does Not Impair hDPC Mineralization and Increases OPN Expression
Calcified nodule formation was examined after 21 and 28 days of differentiation induction. At day 21,
Discussion
Dentin repair beneath the injury sites is strongly dependent on the severity of inflammation within the pulp tissue 14, 15, 16. Therefore, the ideal agent for vital pulp therapy should be able to modulate dental pulp inflammation while promoting repair events. Previous studies have shown that RSG, a PPARγ full agonist, regulates the expression of inflammatory molecules in hDPCs 6, 7. Nevertheless, its role in regulating the cellular events related to dentin repair has not been elucidated. Our
Acknowledgments
The authors thank Bruna Rabelo Amorim for assistance with images editing.
This work was supported by the grants 620195/2008-8 (MCT/CNPq/CT-Infra/CTPetro) and 564666/2010-6 (CNPq/MCT/FAPDF/CAPES), Brazil.
The authors deny any conflicts of interest related to this study.
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