Clinical ResearchDisinfecting Effects of Rotary Instrumentation with Either 2.5% Sodium Hypochlorite or 2% Chlorhexidine as the Main Irrigant: A Randomized Clinical Study
Section snippets
Subjects
The study population consisted of subjects attending the endodontic clinic at the School of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil, for evaluation and treatment of apical periodontitis from March 2009 to December 2010. Only teeth with a single root and a single canal and presenting with carious lesions, necrotic pulps confirmed by pulp tests, and clinical and radiographic evidence of asymptomatic apical periodontitis were included in the study. Reasons for exclusion
Results
Of the 50 individuals included in the study, 27 were male and 23 female, with a mean age of 29 years (range, 13–52 years). All of them reported no significant systemic condition. Each individual contributed 1 tooth.
Bacteria were detected in all samples taken before treatment as revealed by qPCR with universal 16S rRNA gene-based primers. After chemomechanical preparation with rotary instruments and irrigation with either 2.5% NaOCl or 2% CHX, 44% and 40% of the root canals still had detectable
Discussion
The present culture-independent molecular microbiology study was undertaken to evaluate the antibacterial effects of chemomechanical preparation using rotary instrumentation with either 2.5% NaOCl or 2% CHX as the main irrigant in teeth with primary apical periodontitis. Treatment procedures using either solution promoted a substantial reduction in bacterial counts. This is in consonance with many other quantitative culture and molecular analyses of the antibacterial effects of
Acknowledgments
Supported by grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazilian Governmental Institutions.
The authors deny any conflicts of interest related to this study.
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