Regenerative EndodonticsComparative Evaluation of Chemotactic Factor Effect on Migration and Differentiation of Stem Cells of the Apical Papilla
Section snippets
SCAP Culture
A previously characterized SCAP cell line was used (17); cells of passage 4 to 8 were maintained in culture at 37°C and 5% CO2 in basal culture media composed of alpha-minimum essential medium (Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS), 1 L-glutamine (20 μL/mL; Gemini, West Sacramento, CA), penicillin (100 U/mL, Gemini), and streptomycin (100 mg/mL, Gemini) on 10-cm cell culture uncoated polystyrene dishes. Cells were allowed to expand in culture to 70%–80%
SCAP Migration
A kinetic migration experiment was first performed to optimize the time required for maximum migration and the concentration of the FBS to be used. The concentration of 20% FBS evoked the greatest migration observed followed by 10% FBS. On the other hand, 1% and 2% FBS did not evoke an increase in SCAP migration when compared with the negative control (Fig. 1). Importantly, the maximum migration was observed at 24 hours for all the tested groups with no further significant increase in migration
Discussion
The process of cell homing is a normal physiologic event that is closely related to normal wound healing after injury 18, 19. Ideally, reparative stem cells would be recruited into disinfected root canals concomitantly with the progressive formation of supportive blood supply and innervation. This approach would represent an advantage over the existing procedures that bring a substantial number of MSCs to a root canal space devoid of circulation and create mechanical damage of the apical
Acknowledgments
Supported in part by a grant from the American Association of Endodontist Foundation.
The authors deny any conflicts of interest related to this study.
References (50)
- et al.
Regenerative endodontics: a way forward
J Am Dent Assoc
(2016) - et al.
Translational science in disinfection for regenerative endodontics
J Endod
(2014) - et al.
Regenerative endodontic therapy: a data analysis of clinical protocols
J Endod
(2015) - et al.
Evaluation of the delivery of mesenchymal stem cells into the root canal space of necrotic immature teeth after clinical regenerative endodontic procedure
J Endod
(2011) - et al.
Challenges in regenerative endodontics: a case series
J Endod
(2010) - et al.
Continued development of the root separated from the main root
J Endod
(2011) - et al.
Histological findings of revascularized/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma
J Endod
(2013) - et al.
Histologic and histobacteriologic observations of failed revascularization/revitalization therapy: a case report
J Endod
(2014) - et al.
Histologic study of a human immature permanent premolar with chronic apical abscess after revascularization/revitalization
J Endod
(2014) - et al.
Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of the mechano-growth factor prohormone
J Mol Cell Cardiol
(2010)
Mutual, reciprocal SDF-1/CXCR4 interactions between hematopoietic and bone marrow stromal cells regulate human stem cell migration and development in NOD/SCID chimeric mice
Exp Hematol
Dental pulp stem cell migration
J Endod
Characterization of a stem cell of apical papilla cell line: effect of passage on cellular phenotype
J Endod
Chemokine receptors
Cytokine Growth Factor Rev
The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential
Biomaterials
Comparative gene expression analysis of the coronal pulp and apical pulp complex in human immature teeth
J Endod
Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study
J Endod
Histopathological condition of the remaining tissues after endodontic infection of rat immature teeth
J Endod
Age-dependent decline in dental pulp regeneration after pulpectomy in dogs
Exp Gerontol
Mobilized dental pulp stem cells for pulp regeneration: initiation of clinical trial
J Endod
Stromal cell-derived factor-1alpha stimulates tyrosine phosphorylation of multiple focal adhesion proteins and induces migration of hematopoietic progenitor cells: roles of phosphoinositide-3 kinase and protein kinase C
Blood
The expression of stromal cell-derived factor 1 (SDF-1) in inflamed human dental pulp
J Endod
The expression and role of stromal cell-derived factor-1alpha-CXCR4 axis in human dental pulp
J Endod
Regulation of the stromal cell-derived factor-1alpha-CXCR4 axis in human dental pulp cells
J Endod
Inflammation-regeneration interplay in the dentine-pulp complex
J Dent
Cited by (25)
Multiple growth factors accommodated degradable submicron calcium sulfate hemihydrate/porous hydroxyapatite for dentin-pulp regeneration
2022, Biomaterials AdvancesCitation Excerpt :In this study, we synthesized a biodegradable capping material, sCSHA, which can uptake and release multiple GFs (TGF-β1, FGF-2 and VEGF) to facilitate dentin-pulp regeneration potentially. FGF-2 can recruit the mesenchymal stem cells to the lesion site as soon as they are ready in the lesion site [42,43]. The VEGF signal will induce stem cells for the angiogenic differentiation to create blood vessels to transport oxygen and nutrition for further regeneration [44].
bFGF stimulated plasminogen activation factors, but inhibited alkaline phosphatase and SPARC in stem cells from apical Papilla: Involvement of MEK/ERK, TAK1 and p38 signaling
2022, Journal of Advanced ResearchCitation Excerpt :These events may contribute to pericellular proteolysis and the subsequent migration and proliferation of SCAP into the pulp/root canal, which are crucial for clinical success. This can partly explain the stimulation of SCAP migration by bFGF [19,20]. Interestingly, bFGF also stimulated the migration bone marrow and dental pulp stem cells in vitro [47,48], and pulp recellularization and revascularization in endodontically treated human teeth placed into the dorsum of experimental rats in vivo [47].
TGF-β2 and TGF-β1 differentially regulate the odontogenic and osteogenic differentiation of mesenchymal stem cells
2022, Archives of Oral BiologyCitation Excerpt :He et al. reported that TGF-β1 inhibits the expression of DSPP and OCN in SCAPs (He et al., 2014). However, Sara et al. demonstrated that TGF-β1 promotes the mineralization of SCAPs (Sara, Koyo, & Anibal, 2017). Bellamy et al. found that the expression of DSPP and DMP-1 in SCAPs is higher in scaffolds with TGF-β1 than in scaffolds without TGF-β1 (Bellamy, Shrestha, Torneck, & Kishen, 2016).
A pilot study on biological characteristics of human CD24(+) stem cells from the apical papilla
2022, Journal of Dental Sciences