BAPS PaperPatch tracheoplasty in body tissue engineering using collagenous connective tissue membranes (biosheets)
Section snippets
Background
Kimura and associates at Kobe Children's Hospital in Japan first achieved success in patch tracheoplasty in 1982 by insertion into rib cartilage [1]. Re-epithelialization was found at the graft site in every case. However, an ongoing degenerative process was recognized in the graft [2]. Idriss and colleagues in 1984 reported pericardial patch tracheoplasty at the Children's Memorial Hospital in Chicago [3]. In addition, the clinical course of pericardial patch tracheoplasty required tracheal
Materials and methods
All procedures for the present experiments were approved by the animal care committee of Tokyo University (protocol no. P-13-101).
Results
Four weeks after implantation of a silicon tube into the subcutaneous pouch, collagenous connective tissue membranes surrounding the silicone tube were established (Fig. 1a). These connective tissues were white, thin, flexible sheets. In addition, on histological examination, fibrous tissues were recognized by H&E staining (Fig. 1b).
Tracheoplasty using each material was performed without technical difficulty. All rabbits survived and all materials maintained airway structure for 4 weeks after
Discussion
The present study documents that biosheets, which are collagenous connective tissue membranes produced by in-body tissue architecture technology, can maintain airway structure for up to 4 weeks after implantation. The most striking feature of patch tracheoplasty with biosheets was the self-generating capacity of airway cartilage and mucosal epithelium in the region of the patch after 4 weeks of tracheoplasty in a rabbit model. Use of dermis and artificial membranes in patch tracheoplasty could
Acknowledgement
This study was supported by grants from Kawano Masanori Memorial Foundation for Promotion of Pediatrics 2013 and a grant entitled “Study for complex airway reproduction by the new scaffold material using biotube”, JSPS KAKEN Grant Number 25462780. We appreciate the English language review of this article by NAI Inc., Yokohama, Japan.
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