A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

doi:10.1016/j.jviromet.2014.10.006

Journal of Virological Methods

Volume 211, January 2015, Pages 32-35

https://doi.org/10.1016/j.jviromet.2014.10.006 Get rights and content

Highlights

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Using the identity section from RSV-RNA4 and RBSDV-S2 segments to design primers.
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A simple RT-PCR was developed for simultaneous detection of RSV and RBSDV in vector.
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Vector SBPH can carry RSV and RBSDV simultaneously.

Abstract

Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously.

Section snippets

Acknowledgements

This research was supported by the National Natural Science Foundation of China (grant no. 31170142), the Jiangsu Agricultural Scientific Self-innovation Fund (grant no. CX[14]5019) and the Special Fund for Agro-scientific Research in the Public Interest of China (grant no. 201003031).

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Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector

PLOS ONE

(2011)

Cited by (17)

Sensitive and high-throughput polyclonal antibody-based serological methods for rice stripe virus detection in both rice and small brown planthopper
2021, Crop Protection
Citation Excerpt :
To date, numerous detection methods have been developed for RSV. These methods include enzyme-linked immunosorbent assay (ELISA) (Zhen et al., 2004), immunogold labeling (Suzuki et al., 2009), reverse transcription polymerase chain reaction (RT-PCR) (Li et al., 2015; Zhang et al., 2008), reverse transcription loop-mediated isothermal amplification (Le et al., 2010), quantitative RT-PCR (Zhang et al., 2008), and colloidal gold-based immunochromatographic strips (Huang et al., 2019). However, these detection technologies require highly trained staff to operate them; Furthermore, they are suitable only for either rice seedlings or SBPHs, and are not convenient for large-scale detection in the two hosts (rice and SBPHs) in wild fields.
Rice stripe virus (RSV) is a plant virus, and its infection leads to a devastating disease that often causes serious yield losses in rice-producing regions. In this study, the capsid protein (CP) gene of the RSV was cloned and expressed in E. coli (Rosetta), and the purified recombinant His-CP^RSV protein was used to immunize New Zealand white rabbits. After six weeks, a polyclonal antibody against the His-CP^RSV protein (PAb-CP^RSV) was obtained from the serum of the immunized rabbits. Using the prepared PAb-CP^RSV, three serology-based detection methods were developed, which could specifically test for RSV-infected rice plants or small brown planthoppers (SBPHs). Methods that combine dot-blot with enzyme-linked immunosorbent assay to high-throughput methods were also developed to detect RSV in rice seedlings and SBPHs simultaneously in wild fields. The field surveys indicated that the incidence of RSV in rice plants and SBPHs in the suburbs of Yangzhou City was much lower than the value ten years ago. This research presents multiple serology-based RSV detection methods, using the prepared PAb-CP^RSV, which can simultaneously identify RSV-infected rice plants and SBPHs in wild field conditions. Our study will benefit RSV control in rice planting regions.
A simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs
2018, Journal of Virological Methods
Rice black-streaked dwarf virus (RBSDV), an important rice virus, is transmitted by vector small brown planthopper (SBPH) in a persistent manner, but not transovarial transmission. In order to obtain viruliferous SBPH nymphs for relevant research, a simple and reliable method was developed, through allowing SBPH adults laying eggs on RBSDV-infected rice plants. The results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs from the spawning method, which was earlier than insect feed on diseased plant. The average viruliferous rate of SBPH from the spawning method was 32.9%, which was not lower than the feeding diseased plant method. The novel method was very easy to operate and time-saving, facilitating the study on the interaction between RBSDV and SBPH nymphs (especially young 2nd-4th instar nymphs), such as, the effect of RBSDV on nymph development, host plant orientation preference of viruliferous nymph, identification of viral interacting protein in nymph, etc.
Biodiversity of pests and natural enemies in different production environments of rice and cabbage: impacts of insecticide application
2024, Organic Agriculture
Effects of Priming Rice Seeds with Decoyinine on Fitness Traits and Virus Transmission Ability of the Small Brown Planthopper, Laodelphax striatellus
2023, Agronomy
Diversity and Management of Plant Viruses Infecting Rice
2022, Modern Techniques of Rice Crop Production
Genome-wide association study on resistance to rice black-streaked dwarf disease caused by rice black-streaked dwarf virus
2021, Plant Disease

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Journal of Virological Methods

Highlights

Abstract

Section snippets

Acknowledgements

References (22)

One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses

J. Virol. Methods

A one-step real time RT-PCR assay for quantifying rice stripe virus in rice and in the small brown planthopper (Laodelphax striatellus Fallén)

J. Virol. Methods

Occurrence of RSV and RBSDV and their management in Jiangsu Province, P.R. China

Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy

Virol. J.

Biology and molecular biology of viruses in the genus Tenuivirus

Annu. Rev. Phytopathol.

Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies

J. Gen. Virol.

Biology and epidemiology of rice viruses

Annu. Rev. Phytopathol.

Insect vector interactions with persistently transmitted viruses

Annu. Rev. Phytopathol.

Biology and monitoring of vectors in rice stripe epidemiology

Extension Bull.

Characterization of Rice black-streaked dwarf virus- and rice stripe virus-derived siRNAs in singly and doubly infected insect vector Laodelphax striatellus

PLOS ONE

Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector

PLOS ONE

Cited by (17)

Sensitive and high-throughput polyclonal antibody-based serological methods for rice stripe virus detection in both rice and small brown planthopper

A simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs

Biodiversity of pests and natural enemies in different production environments of rice and cabbage: impacts of insecticide application

Effects of Priming Rice Seeds with Decoyinine on Fitness Traits and Virus Transmission Ability of the Small Brown Planthopper, Laodelphax striatellus

Diversity and Management of Plant Viruses Infecting Rice

Genome-wide association study on resistance to rice black-streaked dwarf disease caused by rice black-streaked dwarf virus

Short communicationA simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

Highlights

Abstract

Section snippets

Acknowledgements

J. Virol. Methods

J. Virol. Methods

Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy

Virol. J.

Biology and molecular biology of viruses in the genus Tenuivirus

Annu. Rev. Phytopathol.

Ambisense coding strategy of the rice stripe virus genome: in vitro translation studies

J. Gen. Virol.

Biology and epidemiology of rice viruses

Annu. Rev. Phytopathol.

Insect vector interactions with persistently transmitted viruses

Annu. Rev. Phytopathol.

Biology and monitoring of vectors in rice stripe epidemiology

Extension Bull.

Characterization of Rice black-streaked dwarf virus- and rice stripe virus-derived siRNAs in singly and doubly infected insect vector Laodelphax striatellus

PLOS ONE

Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector

PLOS ONE

Short communication
A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector